Quantitation of Hsp70 levels in Pimephales promelas using a competitive enzyme-linked immunosorbent assay (ELISA) method
- Univ. of North Texas, Denton, TX (United States). Inst. of Applied Sciences
In order to evaluate the expression of stress proteins as biomarkers of the effects of chemical exposure, studies were carried out with Pimephales promelas (fathead minnows). Experiments were designed in which 90 to 120 days old fathead minnows were stressed with varying concentrations (0.25 ppm to 4 ppM) of Cu{sup 2+}, for 4 hours. The levels of Hsp7O in muscle tissue were quantitated by using a competitive enzyme-linked immunosorbent assay (ELISA) method that is highly specific and accurately measures the total pool of Hsp7O in tissues. In previous studies, the concentrations of Hsp7O were quantitated using ELISA in bovine tissue. Hsp70 (for calibration purposes) and antibodies that are specific to constitutive and inducible forms of Hsp7O were purchased commercially. Using this assay method the authors were able to confirm quantitatively, that relative to laboratory controls, higher levels of the inducible form of Hsp70 exists in the tissue of fish that were stressed with Cu{sup 2+}. This method allows for the rapid detection of stress proteins which could be used for field applications.
- OSTI ID:
- 398172
- Report Number(s):
- CONF-9511137--; ISBN 1-880611-03-1
- Country of Publication:
- United States
- Language:
- English
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