Development of bone-lead reference materials for validating in vivo XRF measurements

Journal Article · Sun Dec 31 04:00:00 EST 1995 · Advances in X-Ray Analysis

DOI:https://doi.org/10.1007/978-1-4615-1797-9_74· OSTI ID:369864

Parsons, P J; Zong, Y Y; Matthews, M R ^[1]

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1. SUNY, Albany, NY (United States)

A number of biological reference materials (RM) have been prepared in our laboratory specifically for validating analytical methods for the determination of Pb in biological matrices (e.g. blood, urine, liver, and bone). The RM`s were developed using animal (goats and cows) that are routinely dosed with lead acetate to produce proficiency test samples for blood lead (and erythrocyte protoporphyrin). In cases where an animal becomes injured or infirm, the veterinarian in charge may recommend that the animal be euthanized. In such cases, samples of bone, brain, liver, and other tissues containing lead are removed at autopsy. Currently, we have collected bone samples from nine goats and one cow that were dosed with lead over periods ranging from 1 to 10 years. During the autopsy, the epiphyses (bone joints) are separated from each long bone. Skin, muscle, and other adhering tissues are dissected or scraped from each bone. Bone marrow is also removed. All bare bones are currently stored at -70{degrees}C until analyses for Pb are conducted.

• Sponsoring Organization: USDOE
• OSTI ID: 369864
• Report Number(s): CONF-9408178--
• Journal Information: Advances in X-Ray Analysis, Journal Name: Advances in X-Ray Analysis Vol. 38; ISSN AXRAAA; ISSN 0376-0308
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
66 PHYSICS
ABSORPTION SPECTROSCOPY
BONE TISSUES
CALIBRATION
IN VIVO
LEAD
X-RAY FLUORESCENCE ANALYSIS