Imaging of Vapor Plumes Produced by Matrix Assisted Laser Desorption: A Plume Sharpening Effect
Journal Article
·
· Physical Review Letters
- Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831 (United States)
- General Physics Institute, Russian Academy of Sciences, 117942 Moscow (Russia)
The first gated imaging of both light matrix molecule and heavy biomolecule vapor expansion during matrix assisted laser desorption and ionization is reported, revealing a plume sharpening effect. Laser induced fluorescence imaging of dye-tagged DNasethinspthinspI proteins shows that these heavy molecules (30thinsp000thinspthinspDa) propagate within a very narrow angular distribution compared to that of the 3-HPA matrix (139thinspthinspDa). A special solution of the gas dynamic equations is developed to describe the 3D coexpansion of heavy and light plume components. {copyright} {ital 1999} {ital The American Physical Society}
- Research Organization:
- Oak Ridge National Laboratory
- DOE Contract Number:
- AC05-96OR22464
- OSTI ID:
- 355463
- Journal Information:
- Physical Review Letters, Journal Name: Physical Review Letters Journal Issue: 2 Vol. 83; ISSN 0031-9007; ISSN PRLTAO
- Country of Publication:
- United States
- Language:
- English
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