Spatial and temporal imaging of gas-phase protein and DNA produced by matrix-assisted laser desorption

Journal Article · Tue Feb 01 04:00:00 EST 1994 · Analytical Chemistry (Washington); (United States)

DOI:https://doi.org/10.1021/ac00075a008· OSTI ID:7117200

Heise, T W; Yeung, E S ^[1]

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1. Ames Lab. - USDOE, IA (United States) Iowa State Univ., Ames, IA (United States)

The sensitivity of a transient imaging technique based on laser-excited fluorescence is shown to be within the levels required for studying matrix-assisted laser desorption (MALD) of large biomolecules. Results show that film morphology, particularly film thickness, has a major influence on plume dynamics. Fluorescent labeling of protein and of DNA is used to allow imaging in UV-MALD-generated plumes. Evidence that intact molecules are monitored is provided. Dye molecules, proteins, and DNAs were found to behave very differently in the vaporization process. The spatial and temporal distributions are important for the design of secondary ionization schemes to enhance ion yields, for the optimization of ion collection, and for maximizing resolution in time-of-flight mass spectrometers. 29 refs., 8 figs., 1 tab.

• DOE Contract Number: W-7405-ENG-82
• OSTI ID: 7117200
• Journal Information: Analytical Chemistry (Washington); (United States), Journal Name: Analytical Chemistry (Washington); (United States) Vol. 66:3; ISSN 0003-2700; ISSN ANCHAM
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400102 -- Chemical & Spectral Procedures
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
CHEMICAL ANALYSIS
DESORPTION
DNA
DYES
DYNAMIC MASS SPECTROMETERS
ELECTROMAGNETIC RADIATION
EMISSION SPECTROSCOPY
EVAPORATION
FLUIDS
FLUORESCENCE SPECTROSCOPY
GASES
HEATING
IMAGES
ION SOURCES
IONIZATION
LABELLING
LASER-RADIATION HEATING
MASS SPECTROMETERS
MASS SPECTROSCOPY
MATRIX ISOLATION
MEASURING INSTRUMENTS
NUCLEIC ACIDS
OPTIMIZATION
ORGANIC COMPOUNDS
PHASE TRANSFORMATIONS
PLASMA HEATING
PLUMES
PROTEINS
QUANTITATIVE CHEMICAL ANALYSIS
RADIATIONS
RESOLUTION
SAMPLING
SORPTION
SPATIAL RESOLUTION
SPECTROMETERS
SPECTROSCOPY
TIME RESOLUTION
TIME-OF-FLIGHT MASS SPECTROMETERS
TIME-OF-FLIGHT SPECTROMETERS
TIMING PROPERTIES
ULTRAVIOLET RADIATION
VAPORS