Plume Image Profiling of UV Laser Desorbed Biomolecules
Journal Article
·
· AIP Conference Proceedings
- Centre for Plasma Physics, School of Mathematics and Physics, Queen's University Belfast, Belfast, UK, BT7 1NN (United Kingdom)
- School of Biological Sciences, Queen's University Belfast, Belfast, UK, BT9 7BL (United Kingdom)
- Andor Technology plc., 7 Millennium Way, Springvale Business Park, Belfast, UK, BT12 7AL (United Kingdom)
An experimental system, based upon the techniques of UV and IR laser desorption with time of flight mass spectrometry, has been constructed to enable the production and characterization of neutral biomolecular targets. The feasibility of the laser desorption technique for the purpose of radiation interaction experiments is investigated here. Fluorescent dye tagging and laser induced fluorescence imaging has been used to help characterize the laser produced plumes of biomolecules revealing their spatial density profiles and temporal evolution. Peak target thicknesses of 2x10{sup 12} molecules cm{sup -2} were obtained 30 {mu}s after laser desorption.
- OSTI ID:
- 21254915
- Journal Information:
- AIP Conference Proceedings, Journal Name: AIP Conference Proceedings Journal Issue: 1 Vol. 1080; ISSN APCPCS; ISSN 0094-243X
- Country of Publication:
- United States
- Language:
- English
Similar Records
Spatial and temporal imaging of gas-phase protein and DNA produced by matrix-assisted laser desorption
Imaging of Vapor Plumes Produced by Matrix Assisted Laser Desorption: A Plume Sharpening Effect
Optical characterization of the MALDI plume
Journal Article
·
Mon Jan 31 23:00:00 EST 1994
· Analytical Chemistry (Washington); (United States)
·
OSTI ID:7117200
Imaging of Vapor Plumes Produced by Matrix Assisted Laser Desorption: A Plume Sharpening Effect
Journal Article
·
Thu Jul 01 00:00:00 EDT 1999
· Physical Review Letters
·
OSTI ID:355463
Optical characterization of the MALDI plume
Conference
·
Sat Dec 30 23:00:00 EST 1995
·
OSTI ID:210735