Optical characterization of the MALDI plume
Conference
·
OSTI ID:210735
- Oak Ridge National Lab., TN (United States)
The development of matrix-assisted laser desorption/ionization (MALDI) by Karas, Hillenkamp et al. has brought an entire new class of large-molecule applications within the reach of mass spectrometry. However, the ionization efficiency and therefore the sensitivity of the technique could be improved if details of the ionization mechanism were understood more thoroughly. The environment in the MALDI plume may influence the ionization of the analyte, in analogy to the effect of pH on biomolecules in solution. Optical probing techniques, such as laser-induced fluorescence, allow some characteristics of the plume environment to be studied directly. This paper describes measurement of wavelength-dispersed fluorescence produced in MALDI experiments in a time-of-flight mass spectrometer (TOF-MS).
- OSTI ID:
- 210735
- Report Number(s):
- CONF-9505261--
- Country of Publication:
- United States
- Language:
- English
Similar Records
Fundamental studies of matrix-assisted laser desorption/ionization
Identification of mammalian cell lines using MALDI-TOF and LC-ESI-MS/MS mass spectrometry
Characterization of Microorganisms by MALDI Mass Spectrometry
Conference
·
Fri Dec 30 23:00:00 EST 1994
·
OSTI ID:191706
Identification of mammalian cell lines using MALDI-TOF and LC-ESI-MS/MS mass spectrometry
Journal Article
·
Sat Apr 15 00:00:00 EDT 2006
· Journal of the American Society for Mass Spectrometry
·
OSTI ID:22774061
Characterization of Microorganisms by MALDI Mass Spectrometry
Book
·
Thu Oct 02 00:00:00 EDT 2008
·
OSTI ID:1004031