Optical characterization of the MALDI plume
- Oak Ridge National Lab., TN (United States)
The development of matrix-assisted laser desorption/ionization (MALDI) by Karas, Hillenkamp et al. has brought an entire new class of large-molecule applications within the reach of mass spectrometry. However, the ionization efficiency and therefore the sensitivity of the technique could be improved if details of the ionization mechanism were understood more thoroughly. The environment in the MALDI plume may influence the ionization of the analyte, in analogy to the effect of pH on biomolecules in solution. Optical probing techniques, such as laser-induced fluorescence, allow some characteristics of the plume environment to be studied directly. This paper describes measurement of wavelength-dispersed fluorescence produced in MALDI experiments in a time-of-flight mass spectrometer (TOF-MS).
- OSTI ID:
- 210735
- Report Number(s):
- CONF-9505261--
- Country of Publication:
- United States
- Language:
- English
Similar Records
Identification of mammalian cell lines using MALDI-TOF and LC-ESI-MS/MS mass spectrometry
Characterization of Microorganisms by MALDI Mass Spectrometry