LIF-imaging and gas-phase diagnostics of laser desorbed MALDI-matrix plumes

Technical Report · Tue Jul 01 04:00:00 EDT 1997

DOI:https://doi.org/10.2172/563317· OSTI ID:563317

Puretzky, A A; Geohegan, D B ^[1]

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1. Oak Ridge National Lab., TN (United States). Solid State Div.

The first gated LIF-imaging and absorption spectroscopy has been performed on laser desorbed plumes from organic crystals which are commonly used as MALDI (Matrix Assisted Laser Desorption Ionization) matrices. These plasma diagnostic techniques, including ion probe measurements were employed to investigate the desorbed products, densities, fractional ionization, and velocity distributions of the plume of ejecta which is typically employed as the main desorption product in the mass spectrometry analysis of large biomolecules. Ultraviolet pulsed 193-nm and 248-nm irradiation of 3-hydroxypicolinic acid (3-HPA) crystals were studied to understand the effect of very different gas-phase absorption cross sections measured here for this material. In both cases, LIF imaging revealed two plume components: a fast (maximum {approximately} 0.1 cm/{micro}s) low-intensity component which appear to be 3-HPA fragments, and a slower component of 3-HPA expanding at 0.05 cm/{micro}s. In the case of ArF-laser irradiation, optical absorption spectroscopy indicated a breaking of the intramolecular hydrogen bond in the gas-phase matrix material.

• Research Organization: Oak Ridge National Lab., TN (United States)
• Sponsoring Organization: USDOE Office of Energy Research, Washington, DC (United States)
• DOE Contract Number: AC05-96OR22464
• OSTI ID: 563317
• Report Number(s): CONF-9707110--1; ON: DE97009382; BR: KC0202020
• Country of Publication: United States
• Language: English

Related Subjects

40 CHEMISTRY
ABSORPTION SPECTROSCOPY
BIOLOGICAL MATERIALS
EXPERIMENTAL DATA
FLUORESCENCE
LASER RADIATION
MASS SPECTROSCOPY
PLASMA DIAGNOSTICS
TIME-OF-FLIGHT MASS SPECTROMETERS