Assignment of the human diacylglycerol kinase gene (DAGK) to 12q13.3 using fluorescence in situ hybridization analysis
- Eastman Dental Center, Rochester, NY (United States); and others
A 1-kb cDNA probe specific for the human DAGK gene was prepared by polymerase chain reaction and used to assign it to human chromosome 12 using a human x hamster somatic cell hybrid mapping panel. To determine the chromosomal sublocalization of DAGK, used the 1-kg DAGK cDNA probe to isolate a DAGK-specific phage clone from a human chromosome 12 library by Southern blot techniques. This clone (phEDCDAGK) contained a 17-kb human genomic insert in the phage Charon 40 that hybridized to the 1-kb DAGK cDNA previously characterized.
- OSTI ID:
- 255194
- Journal Information:
- Genomics, Journal Name: Genomics Journal Issue: 1 Vol. 22; ISSN 0888-7543; ISSN GNMCEP
- Country of Publication:
- United States
- Language:
- English
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