Assignment of the human ubiquitous receptor gene (UNR) to 19q13.3 using fluorescence in situ hybridization
- Univ. of Chicago, IL (United States); and others
Human UNR cDNAs were used to screen a Lambda FIX II human male placenta genomic library. Phage DNA from clones hybridizing to UNR cDNA was characterized by Southern hybridization and restriction mapping, and two different clones (hG10 and hG12) with inserts of 15-20 kb were chosen for fluorescence in situ hybridization (FISH) analysis. Biotin-labeled probes were prepared from phage DNA by nick-translation using Bio-11-dUTP. FISH was performed as described previously. Hybridization was detected with fluorescien-conjugated avidin, and chromosomes were identified by staining with 4,6-diamidino-2-phenylindole-dihydrochloride (DAPI).
- OSTI ID:
- 249979
- Journal Information:
- Genomics, Journal Name: Genomics Journal Issue: 1 Vol. 26; ISSN 0888-7543; ISSN GNMCEP
- Country of Publication:
- United States
- Language:
- English
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