Assignment of the human pro alpha 2(I) collagen structural gene (COLIA2) to chromosome 7 by molecular hybridization
Journal Article
·
· Am. J. Hum. Genet.; (United States)
OSTI ID:6816723
A cDNA for the pro alpha 2 chain of human type I collagen has been recently cloned and amplified. Researchers have used this specific probe to identify the human chromosome carrying the pro alpha 2(I) collagen gene. The DNA from 17 independent human/hamster and human/mouse somatic cell hybrids was digested by Eco RI and the restriction pattern analyzed in Southern blot experiments, using the /sup 32/P-labeled cDNA as a hybridization probe. The gene coding for the pro alpha 2 collagen subunit could be unambiguously assigned to human chromosome 7. All the other chromosomes, including chromosome 17, were excluded.
- Research Organization:
- Institut de Pathologie Moleculaire, INSERM U 129, Paris, France
- OSTI ID:
- 6816723
- Journal Information:
- Am. J. Hum. Genet.; (United States), Journal Name: Am. J. Hum. Genet.; (United States) Vol. 34:3; ISSN AJHGA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201 -- Biochemistry-- Tracer Techniques
550401 -- Genetics-- Tracer Techniques
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
CHEMISTRY
CHROMOSOMES
COLLAGEN
DAYS LIVING RADIOISOTOPES
DNA
GENES
GENETIC MAPPING
ISOTOPES
LIGHT NUCLEI
MAMMALS
MAN
MAPPING
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PRIMATES
PROTEINS
RADIOISOTOPES
SCLEROPROTEINS
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
550401 -- Genetics-- Tracer Techniques
550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
CHEMISTRY
CHROMOSOMES
COLLAGEN
DAYS LIVING RADIOISOTOPES
DNA
GENES
GENETIC MAPPING
ISOTOPES
LIGHT NUCLEI
MAMMALS
MAN
MAPPING
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PRIMATES
PROTEINS
RADIOISOTOPES
SCLEROPROTEINS
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES