Golgi-localized putative S-adenosyl methionine transporters required for plant cell wall polysaccharide methylation

Temple, Henry; Phyo, Pyae; Yang, Weibing; Lyczakowski, Jan J.; Echevarría-Poza, Alberto; Yakunin, Igor; Parra-Rojas, Juan Pablo; Terrett, Oliver M.; Saez-Aguayo, Susana; Dupree, Ray; Orellana, Ariel; Hong, Mei; Dupree, Paul

doi:10.1038/s41477-022-01156-4

Golgi-localized putative S-adenosyl methionine transporters required for plant cell wall polysaccharide methylation

Journal Article · Thu Jun 09 00:00:00 EDT 2022 · Nature Plants (Online)

DOI:https://doi.org/10.1038/s41477-022-01156-4· OSTI ID:1883363

^[1]; Phyo, Pyae ^[2]; Yang, Weibing ^[3]; Lyczakowski, Jan J. ^[4]; Echevarría-Poza, Alberto ^[5]; ^[5]; Parra-Rojas, Juan Pablo ^[6]; Terrett, Oliver M. ^[5]; Saez-Aguayo, Susana ^[6]; Dupree, Ray ^[7]; Orellana, Ariel ^[6]; ^[2]; ^[5]

Univ. of Cambridge (United Kingdom); University of Cambridge
Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States)
Chinese Academy of Sciences (CAS) and CAS-JIC Center of Excellence for Plant and Microbial Sciences (CEPAMS), Shanghai (China); Univ. of Cambridge (United Kingdom)
Univ. of Cambridge (United Kingdom); Jagiellonian Univ., Krakow (Poland)
Univ. of Cambridge (United Kingdom)
Univ. Andrés Bello, Santiago (Chile)
Univ. of Warwick, Coventry (United Kingdom)

Polysaccharide methylation, especially that of pectin, is a common and important feature of land plant cell walls. Polysaccharide methylation takes place in the Golgi apparatus and therefore relies on the import of S-adenosyl methionine (SAM) from the cytosol into the Golgi. However, so far, no Golgi SAM transporter has been identified in plants. Here we studied major facilitator superfamily members in Arabidopsis that we identified as putative Golgi SAM transporters (GoSAMTs). Knockout of the two most highly expressed GoSAMTs led to a strong reduction in Golgi-synthesized polysaccharide methylation. Furthermore, solid-state NMR experiments revealed that reduced methylation changed cell wall polysaccharide conformations, interactions and mobilities. Notably, NMR revealed the existence of pectin ‘egg-box’ structures in intact cell walls and showed that their formation is enhanced by reduced methyl esterification. Here, these changes in wall architecture were linked to substantial growth and developmental phenotypes. In particular, anisotropic growth was strongly impaired in the double mutant. The identification of putative transporters involved in import of SAM into the Golgi lumen in plants provides new insights into the paramount importance of polysaccharide methylation for plant cell wall structure and function.

View Accepted Manuscript (DOE)

Research Organization:: Pennsylvania State Univ., University Park, PA (United States). Center for Lignocellulose Structure and Formation

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: SC0001090

OSTI ID:: 1883363

Journal Information:: Nature Plants (Online), Journal Name: Nature Plants (Online) Journal Issue: 6 Vol. 8; ISSN 2055-0278

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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