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QUASIMODO 3 (QUA3) is a putative homogalacturonan methyltransferase regulating cell wall biosynthesis in Arabidopsis suspension-cultured cells

Journal Article · · Journal of Experimental Botany
DOI:https://doi.org/10.1093/jxb/err211· OSTI ID:1625374
 [1];  [2];  [2];  [2];  [2]
  1. The Chinese Univ. of Hong Kong (China). Centre for Cell and Developmental Biology. School of Life Sciences; DOE/OSTI
  2. The Chinese Univ. of Hong Kong (China). Centre for Cell and Developmental Biology. School of Life Sciences
+ Show Author Affiliations
Pectins are complex polysaccharides that are essential components of the plant cell wall. In this study, a novel putative Arabidopsis S-adenosyl-L-methionine (SAM)-dependent methyltransferase, termed QUASIMODO 3 (QUA3, At4g00740), has been characterized and it was demonstrated that it is a Golgi-localized, type II integral membrane protein that functions in methylesterification of the pectin homogalacturonan (HG). Although transgenic Arabidopsis seedlings with overexpression, or knock-down, of QUA3 do not show altered phenotypes or changes in pectin methylation, this enzyme is highly expressed and abundant in Arabidopsis suspension-cultured cells. In contrast, in cells subjected to QUA3 RNA interference (RNAi) knock-down there is less pectin methylation as well as altered composition and assembly of cell wall polysaccharides. Taken together, these observations point to a Golgi-localized QUA3 playing an essential role in controlling pectin methylation and cell wall biosynthesis in Arabidopsis suspension cell cultures.
Research Organization:
Univ. of Georgia, Athens, GA (United States)
Sponsoring Organization:
USDOE Office of Science (SC)
Grant/Contract Number:
FG02-93ER20097; FG05-93ER20097
OSTI ID:
1625374
Journal Information:
Journal of Experimental Botany, Journal Name: Journal of Experimental Botany Journal Issue: 14 Vol. 62; ISSN 0022-0957
Publisher:
Oxford University PressCopyright Statement
Country of Publication:
United States
Language:
English

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Successful transport to the vacuole of heterologously expressed mung bean 8S globulin occurs in seed but not in vegetative tissues journal February 2013
ARA7(Q69L) expression in transgenic Arabidopsis cells induces the formation of enlarged multivesicular bodies journal May 2013
Golgi-Mediated Synthesis and Secretion of Matrix Polysaccharides of the Primary Cell Wall of Higher Plants journal January 2012
Additional file 1 of Characterization of novel loci controlling seed oil content in Brassica napus by marker metabolite-based multi-omics analysis dataset January 2023
Plant cell wall integrity maintenance in model plants and crop species-relevant cell wall components and underlying guiding principles journal November 2019
IbSIMT1, a novel salt-induced methyltransferase gene from Ipomoea batatas, is involved in salt tolerance journal October 2014
Dissection of allelic interactions among Pto-miR257 and its targets and their effects on growth and wood properties in Populus journal April 2016
Galacturonosyltransferase (GAUT)1 and GAUT7 are the core of a plant cell wall pectin biosynthetic homogalacturonan:galacturonosyltransferase complex journal November 2011
Membrane topology of Golgi-localized probable S-adenosylmethionine-dependent methyltransferase in tobacco ( Nicotiana tabacum ) BY-2 cells journal December 2015
We be jammin’: an update on pectin biosynthesis, trafficking and dynamics journal November 2015
Trans‐methylation reactions in plants: focus on the activated methyl cycle journal October 2017

Figures / Tables (12)

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Related Subjects

54 ENVIRONMENTAL SCIENCES
Arabidopsis
Plant Sciences
S-adenosyl-Lmethionine
cell wall
homogalacturonan
methyltransferase
pectin
plant suspension cultured cells