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UUAT1 Is a Golgi-Localized UDP-Uronic Acid Transporter That Modulates the Polysaccharide Composition of Arabidopsis Seed Mucilage

Journal Article · · The Plant Cell
DOI:https://doi.org/10.1105/tpc.16.00465· OSTI ID:1393109
 [1];  [2];  [1];  [1];  [1];  [1];  [1];  [1];  [2];  [3];  [3];  [4];  [5];  [6];  [1];  [1]
  1. Univ. of Adres Bello, Santiago (Chile). Center for Plant Biotechnology
  2. Univ. of Melbourne (Australia). ARC Centre of Excellence in Plant Cell Walls
  3. Normandy Univ. (France)
  4. Univ. of Cambridge (United Kingdom). Dept. of Biochemistry
  5. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Joit BioEnergy Inst. and Biological Systems and Engineering Division; Univ. of California, Berkeley, CA (United States). Dept. of Plant and Microbial Biology
  6. Univ. of Melbourne (Australia). ARC Centre of Excellence in Plant Cell Walls; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Joint BioEnergy Inst. and Biological Systems and Engineering Division
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UDP-glucuronic acid (UDP-GlcA) is the precursor of many plant cell wall polysaccharides and is required for production of seed mucilage. Following synthesis in the cytosol, it is transported into the lumen of the Golgi apparatus, where it is converted to UDP-galacturonic acid (UDP-GalA), UDP-arabinose, and UDP-xylose. To identify the Golgi-localized UDP-GlcA transporter, we screened Arabidopsis thaliana mutants in genes coding for putative nucleotide sugar transporters for altered seed mucilage, a structure rich in the GalA-containing polysaccharide rhamnogalacturonan I. As a result, we identified UUAT1, which encodes a Golgi-localized protein that transports UDP-GlcA and UDP-GalA in vitro. The seed coat of uuat1 mutants had less GalA, rhamnose, and xylose in the soluble mucilage, and the distal cell walls had decreased arabinan content. Cell walls of other organs and cells had lower arabinose levels in roots and pollen tubes, but no differences were observed in GalA or xylose contents. Furthermore, the GlcA content of glucuronoxylan in the stem was not affected in the mutant. Interestingly, the degree of homogalacturonan methylation increased in uuat1. These results suggest that this UDP-GlcA transporter plays a key role defining the seed mucilage sugar composition and that its absence produces pleiotropic effects in this component of the plant extracellular matrix.
Research Organization:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
Grant/Contract Number:
AC02-05CH11231
OSTI ID:
1393109
Journal Information:
The Plant Cell, Journal Name: The Plant Cell Journal Issue: 1 Vol. 29; ISSN 1040-4651
Publisher:
American Society of Plant BiologistsCopyright Statement
Country of Publication:
United States
Language:
English

References (10)

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Cited By (13)

Three UDP-xylose transporters participate in xylan biosynthesis by conveying cytosolic UDP-xylose into the Golgi lumen in Arabidopsis journal December 2017
ICE 1 and ZOU determine the depth of primary seed dormancy in Arabidopsis independently of their role in endosperm development journal February 2019
UDP-Glucuronic Acid Transport Is Required for Virulence of Cryptococcus neoformans journal January 2018
Chlorophyll Fluorescence Video Imaging: A Versatile Tool for Identifying Factors Related to Photosynthesis journal January 2018
Xylan in the Middle: Understanding Xylan Biosynthesis and Its Metabolic Dependencies Toward Improving Wood Fiber for Industrial Processing journal February 2019
Co-Expression Network Analysis and Hub Gene Selection for High-Quality Fiber in Upland Cotton (Gossypium hirsutum) Using RNA Sequencing Analysis journal February 2019
A Toolkit for the Characterization of the Photoprotective Capacity of Green Algae book January 2018
A Golgi UDP-GlcNAc transporter delivers substrates for N-linked glycans and sphingolipids journal September 2018
Conserved Glu-47 and Lys-50 residues are critical for UDP- N -acetylglucosamine/UMP antiport activity of the mouse Golgi-associated transporter Slc35a3 journal May 2019
A hypomorphic allele of SLC35D1 results in Schneckenbecken-like dysplasia journal August 2019
Secondary cell wall biosynthesis journal November 2018
Golgi-localized cation/proton exchangers regulate ionic homeostasis and skotomorphogenesis in Arabidopsis: Golgi-Localized KEAs Regulate Ionic Homeostasis journal November 2018
Bifunctional glycosyltransferases catalyze both extension and termination of pectic galactan oligosaccharides journal March 2018

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES