Advanced genetic tools enable synthetic biology in the oleaginous microalgae Nannochloropsis sp.

Poliner, Eric; Farré, Eva M.; Benning, Christoph

doi:10.1007/s00299-018-2270-0

Advanced genetic tools enable synthetic biology in the oleaginous microalgae Nannochloropsis sp.

Journal Article · Tue Mar 06 00:00:00 EST 2018 · Plant Cell Reports

DOI:https://doi.org/10.1007/s00299-018-2270-0· OSTI ID:1608420

Poliner, Eric ^[1]; Farré, Eva M. ^[2]; ^[2]

Michigan State Univ., East Lansing, MI (United States); MSU DOE Plant Research Labarotory
Michigan State Univ., East Lansing, MI (United States)

Nannochloropsis is a genus of fast-growing microalgae that are regularly used for biotechnology applications. Nannochloropsis species have a high triacylglycerol content and their polar lipids are rich in the omega-3 long-chain polyunsaturated fatty acid, eicosapentaenoic acid. Placed in the heterokont lineage, the Nannochloropsis genus has a complex evolutionary history. Genome sequences are available for several species, and a number of transcriptomic datasets have been produced, making this genus a facile model for comparative genomics. There is a growing interest in Nannochloropsis species as models for the study of microalga lipid metabolism and as a chassis for synthetic biology. Recently, techniques for gene stacking, and targeted gene disruption and repression in the Nannochloropsis genus have been developed. These tools enable gene-specific, mechanistic studies and have already allowed the engineering of improved Nannochloropsis strains with superior growth, or greater bioproduction.

View Accepted Manuscript (DOE)

Research Organization:: Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Laboratory

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)

Grant/Contract Number:: FG02-91ER20021

OSTI ID:: 1608420

Journal Information:: Plant Cell Reports, Journal Name: Plant Cell Reports Journal Issue: 10 Vol. 37; ISSN 0721-7714

Publisher:: Springer NatureCopyright Statement

Country of Publication:: United States

Language:: English

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Transcriptional coordination of physiological responses in Nannochloropsis oceanica CCMP1779 under light/dark cycles Poliner, Eric; Panchy, Nicholas; Newton, Linsey The Plant Journal, Vol. 83, Issue 6 https://doi.org/10.1111/tpj.12944	journal	August 2015
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RNA Interference Silencing of a Major Lipid Droplet Protein Affects Lipid Droplet Size in Chlamydomonas reinhardtii Moellering, Eric R.; Benning, Christoph Eukaryotic Cell, Vol. 9, Issue 1 https://doi.org/10.1128/EC.00203-09	journal	November 2009
The Response of Nannochloropsis gaditana to Nitrogen Starvation Includes De Novo Biosynthesis of Triacylglycerols, a Decrease of Chloroplast Galactolipids, and Reorganization of the Photosynthetic Apparatus Simionato, Diana; Block, Maryse A.; La Rocca, Nicoletta Eukaryotic Cell, Vol. 12, Issue 5 https://doi.org/10.1128/EC.00363-12	journal	March 2013
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Genome, Functional Gene Annotation, and Nuclear Transformation of the Heterokont Oleaginous Alga Nannochloropsis oceanica CCMP1779 Vieler, Astrid; Wu, Guangxi; Tsai, Chia-Hong PLoS Genetics, Vol. 8, Issue 11 https://doi.org/10.1371/journal.pgen.1003064	journal	November 2012
Nannochloropsis Genomes Reveal Evolution of Microalgal Oleaginous Traits Wang, Dongmei; Ning, Kang; Li, Jing PLoS Genetics, Vol. 10, Issue 1 https://doi.org/10.1371/journal.pgen.1004094	journal	January 2014
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