Engineering whiting events in culture: A microalgae-driven calcium carbonate and biomass production process at high pH and alkalinity with the marine microalga Nannochloropsis oceanica IMET1

Lee, Yi-Ying; Jonas, Lauren; Hill, Russell; Place, Allen; Silsbe, Greg; Hunsicker, Scott; North, Elizabeth; Li, Yantao

doi:10.1016/j.jcou.2024.102669

Engineering whiting events in culture: A microalgae-driven calcium carbonate and biomass production process at high pH and alkalinity with the marine microalga Nannochloropsis oceanica IMET1

Journal Article · Thu Jan 18 00:00:00 EST 2024 · Journal of CO2 Utilization

DOI:https://doi.org/10.1016/j.jcou.2024.102669· OSTI ID:3013945

Lee, Yi-Ying ^[1]; Jonas, Lauren ^[1]; Hill, Russell ^[1]; Place, Allen ^[1]; Silsbe, Greg ^[1]; Hunsicker, Scott ^[1]; North, Elizabeth ^[1]; Li, Yantao ^[2]

University of Maryland Center for Environmental Science, Baltimore, MD (United States)
University of Maryland Center for Environmental Science, Baltimore, MD (United States); University of Maryland Baltimore County, MD (United States)

The impact of whiting events on global inorganic carbon cycle and climate change is debatable at the current ocean pH. This work engineered whiting events in a high pH, high alkalinity microalgal culture for carbon dioxide capture and storage. When growing the marine microalga Nannochloropsis oceanica IMET1 in photobioreactors, culture alkalinity more than doubled, increasing from 72.5 mg L^-1 to a maximum level of 159.6 mg L^-1. At the same time, culture pH increased from 7.9 to 10.1 with concomitant calcium carbonate production. X-ray diffraction analysis revealed the precipitated calcium carbonate was primarily monohydrocalcite. The lab culture was scaled up to a 340-L bioreactor, in which Nannochloropsis ash-free dry weight productivities ranged from 25.1 to 51.4 g m^-2 d^-1 and a maximum monohydrocalcite productivity of 133.4 g m² d^-1 was recorded. In this system, Nannochloropsis biomass contained about 23.9 % lipids and the eicosapentaenoic acid content was about 1.8 %. Together, these results suggest the microalgae-driven calcium carbonate and biomass production process efficiently captures and stores atmospheric carbon dioxide in the form of calcium carbonate while producing valuable bioproducts. Study of the bacterial communities associated with the Nannochloropsis culture identified four dominant species Maricaulis maris, Marinisubtilis pacificus, Gracilimonas sp., and an uncultured bacterium in the OD1 phylum with interesting features that warrant further investigation.

View Accepted Manuscript (DOE)

Research Organization:: Univ. of Maryland, College Park, MD (United States). Center for Environmental Science; University of Maryland Baltimore County, MD (United States); University of Maryland Center for Environmental Science, Baltimore, MD (United States)

Sponsoring Organization:: USDOE Office of Fossil Energy (FE); USDOE Office of Fossil Energy and Carbon Management (FECM)

Grant/Contract Number:: FE0031914; FE0032188

OSTI ID:: 3013945

Alternate ID(s):: OSTI ID: 2576744

Report Number(s):: DOE-UMCES--31914

Journal Information:: Journal of CO2 Utilization, Journal Name: Journal of CO2 Utilization Vol. 80; ISSN 2212-9820

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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Engineering whiting events in culture: A microalgae-driven calcium carbonate and biomass production process at high pH and alkalinity with the marine microalga Nannochloropsis oceanica IMET1

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