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Indole Glucosinolate Biosynthesis Limits Phenylpropanoid Accumulation in Arabidopsis thaliana

Journal Article · · The Plant Cell
DOI:https://doi.org/10.1105/tpc.15.00127· OSTI ID:1597755
 [1];  [2];  [2];  [2]
  1. Purdue Univ., West Lafayette, IN (United States). Dept. of Biochemistry; Purdue University
  2. Purdue Univ., West Lafayette, IN (United States). Dept. of Biochemistry
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Plants produce an array of metabolites (including lignin monomers and soluble UV-protective metabolites) from phenylalanine through the phenylpropanoid biosynthetic pathway. A subset of plants, including many related to Arabidopsis thaliana, synthesizes glucosinolates, nitrogen- and sulfur-containing secondary metabolites that serve as components of a plant defense system that deters herbivores and pathogens. Here, we report that the Arabidopsis thaliana reduced epidermal fluorescence5 (ref5-1) mutant, identified in a screen for plants with defects in soluble phenylpropanoid accumulation, has a missense mutation in CYP83B1 and displays defects in glucosinolate biosynthesis and in phenylpropanoid accumulation. CYP79B2 and CYP79B3 are responsible for the production of the CYP83B1 substrate indole-3-acetaldoxime (IAOx), and we found that the phenylpropanoid content of cyp79b2 cyp79b3 and ref5-1 cyp79b2 cyp79b3 plants is increased compared with the wild type. These data suggest that levels of IAOx or a subsequent metabolite negatively influence phenylpropanoid accumulation in ref5 and more importantly that this crosstalk is relevant in the wild type. Additional biochemical and genetic evidence indicates that this inhibition impacts the early steps of the phenylpropanoid biosynthetic pathway and restoration of phenylpropanoid accumulation in a ref5-1 med5a/b triple mutant suggests that the function of the Mediator complex is required for the crosstalk.

Research Organization:
Purdue Univ., West Lafayette, IN (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
Grant/Contract Number:
FG02-07ER15905
OSTI ID:
1597755
Alternate ID(s):
OSTI ID: 1210812
OSTI ID: 1385850
Journal Information:
The Plant Cell, Journal Name: The Plant Cell Journal Issue: 5 Vol. 27; ISSN 1040-4651
Publisher:
American Society of Plant BiologistsCopyright Statement
Country of Publication:
United States
Language:
English

Cited By (13)

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UVA, UVB Light, and Methyl Jasmonate, Alone or Combined, Redirect the Biosynthesis of Glucosinolates, Phenolics, Carotenoids, and Chlorophylls in Broccoli Sprouts journal November 2017
Roles of Plant-Derived Secondary Metabolites during Interactions with Pathogenic and Beneficial Microbes under Conditions of Environmental Stress journal September 2019
A noninvasive, machine learning–based method for monitoring anthocyanin accumulation in plants using digital color imaging journal November 2019
The molecular genetic basis of herbivory between butterflies and their host plants journal August 2018
Arabidopsis myrosinases link the glucosinolate-myrosinase system and the cuticle journal December 2016
Epigenetic mapping of the Arabidopsis metabolome reveals mediators of the epigenotype-phenotype map journal November 2018
Combined transcriptome and translatome analyses reveal a role for tryptophan-dependent auxin biosynthesis in the control of DOG1 -dependent seed dormancy journal November 2017
Mutation of Mediator subunit CDK 8 counteracts the stunted growth and salicylic acid hyperaccumulation phenotypes of an Arabidopsis MED 5 mutant journal March 2019
Glucosinolate and phenylpropanoid biosynthesis are linked by proteasome‐dependent degradation of PAL journal September 2019
Dynamic metabolic and transcriptomic profiling of methyl jasmonate‐treated hairy roots reveals synthetic characters and regulators of lignan biosynthesis in Isatis indigotica Fort journal June 2016
The Arabidopsis thaliana Knockout Mutant for Phytochelatin Synthase1 (cad1-3) Is Defective in Callose Deposition, Bacterial Pathogen Defense and Auxin Content, But Shows an Increased Stem Lignification journal January 2018

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59 BASIC BIOLOGICAL SCIENCES