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Title: Glucosinolate and phenylpropanoid biosynthesis are linked by proteasome-dependent degradation of PAL

Abstract

Plants produce several hundreds of thousands of secondary metabolites that are important for adaptation to various environmental conditions. Although different groups of secondary metabolites are synthesized through unique biosynthetic pathways, plants must orchestrate their production simultaneously. Phenylpropanoids and glucosinolates are two classes of secondary metabolites that are synthesized through apparently independent biosynthetic pathways. Genetic evidence has revealed that the accumulation of glucosinolate intermediates limits phenylpropanoid production in a Mediator Subunit 5 (MED5)-dependent manner. To elucidate the molecular mechanism underlying this process, we analyzed the transcriptomes of a suite of Arabidopsis thaliana glucosinolate-deficient mutants using RNAseq and identified misregulated genes that are rescued by the disruption of MED5. The expression of a group of Kelch Domain F-Box genes (KFBs) that function in PAL degradation is affected in glucosinolate biosynthesis mutants and the disruption of these KFBs restores phenylpropanoid deficiency in the mutants. Our study suggests that glucosinolate/phenylpropanoid metabolic crosstalk involves the transcriptional regulation of KFB genes that initiate the degradation of the enzyme phenylalanine ammonia-lyase, which catalyzes the first step of the phenylpropanoid biosynthesis pathway. Nevertheless, KFB mutant plants remain partially sensitive to glucosinolate pathway mutations, suggesting that other mechanisms that link the two pathways also exist.

Authors:
; ; ; ;
Publication Date:
Research Org.:
Purdue Univ., West Lafayette, IN (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
OSTI Identifier:
1597911
Alternate Identifier(s):
OSTI ID: 1561330
Grant/Contract Number:  
FG02-07ER15905; SC0012704; SC0000997; DE‐SC0000997
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
New Phytologist
Additional Journal Information:
Journal Volume: 225; Journal Issue: 1; Journal ID: ISSN 0028-646X
Publisher:
Wiley
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Kim, Jeong Im, Zhang, Xuebin, Pascuzzi, Pete, Liu, Chang-Jun, and Chapple, Clint. Glucosinolate and phenylpropanoid biosynthesis are linked by proteasome-dependent degradation of PAL. United States: N. p., 2019. Web. doi:10.1111/nph.16108.
Kim, Jeong Im, Zhang, Xuebin, Pascuzzi, Pete, Liu, Chang-Jun, & Chapple, Clint. Glucosinolate and phenylpropanoid biosynthesis are linked by proteasome-dependent degradation of PAL. United States. doi:10.1111/nph.16108.
Kim, Jeong Im, Zhang, Xuebin, Pascuzzi, Pete, Liu, Chang-Jun, and Chapple, Clint. Wed . "Glucosinolate and phenylpropanoid biosynthesis are linked by proteasome-dependent degradation of PAL". United States. doi:10.1111/nph.16108. https://www.osti.gov/servlets/purl/1597911.
@article{osti_1597911,
title = {Glucosinolate and phenylpropanoid biosynthesis are linked by proteasome-dependent degradation of PAL},
author = {Kim, Jeong Im and Zhang, Xuebin and Pascuzzi, Pete and Liu, Chang-Jun and Chapple, Clint},
abstractNote = {Plants produce several hundreds of thousands of secondary metabolites that are important for adaptation to various environmental conditions. Although different groups of secondary metabolites are synthesized through unique biosynthetic pathways, plants must orchestrate their production simultaneously. Phenylpropanoids and glucosinolates are two classes of secondary metabolites that are synthesized through apparently independent biosynthetic pathways. Genetic evidence has revealed that the accumulation of glucosinolate intermediates limits phenylpropanoid production in a Mediator Subunit 5 (MED5)-dependent manner. To elucidate the molecular mechanism underlying this process, we analyzed the transcriptomes of a suite of Arabidopsis thaliana glucosinolate-deficient mutants using RNAseq and identified misregulated genes that are rescued by the disruption of MED5. The expression of a group of Kelch Domain F-Box genes (KFBs) that function in PAL degradation is affected in glucosinolate biosynthesis mutants and the disruption of these KFBs restores phenylpropanoid deficiency in the mutants. Our study suggests that glucosinolate/phenylpropanoid metabolic crosstalk involves the transcriptional regulation of KFB genes that initiate the degradation of the enzyme phenylalanine ammonia-lyase, which catalyzes the first step of the phenylpropanoid biosynthesis pathway. Nevertheless, KFB mutant plants remain partially sensitive to glucosinolate pathway mutations, suggesting that other mechanisms that link the two pathways also exist.},
doi = {10.1111/nph.16108},
journal = {New Phytologist},
issn = {0028-646X},
number = 1,
volume = 225,
place = {United States},
year = {2019},
month = {9}
}

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