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Loss of ferulate 5-hydroxylase leads to Mediator-dependent inhibition of soluble phenylpropanoid biosynthesis in Arabidopsis

Journal Article · · Plant Physiology (Bethesda)
DOI:https://doi.org/10.1104/pp.15.00294· OSTI ID:1386267
 [1];  [2];  [3];  [4]
  1. Purdue Univ., West Lafayette, IN (United States); Heartland Plant Innovations, Manhattan, KS (United States)
  2. Purdue Univ., West Lafayette, IN (United States); Dow AgroSciences LLC, Indianapolis, IN (United States)
  3. Purdue Univ., West Lafayette, IN (United States); Univ. of Wisconsin, Madison, WI (United States)
  4. Purdue Univ., West Lafayette, IN (United States)
+ Show Author Affiliations

Phenylpropanoids are phenylalanine-derived specialized metabolites and include important structural components of plant cell walls, such as lignin and hydroxycinnamic acids, as well as ultraviolet and visible light-absorbing pigments, such as hydroxycinnamate esters (HCEs) and anthocyanins. Previous work has revealed a remarkable degree of plasticity in HCE biosynthesis, such that most Arabidopsis (Arabidopsis thaliana) mutants with blockages in the pathway simply redirect carbon flux to atypical HCEs. In contrast, the ferulic acid hydroxylase1 (fah1) mutant accumulates greatly reduced levels of HCEs, suggesting that phenylpropanoid biosynthesis may be repressed in response to the loss of FERULATE 5-HYDROXYLASE (F5H) activity. Here, we show that in fah1 mutant plants, the activity of HCE biosynthetic enzymes is not limiting for HCE accumulation, nor is phenylpropanoid flux diverted to the synthesis of cell wall components or flavonol glycosides. We further show that anthocyanin accumulation is also repressed in fah1 mutants and that this repression is specific to tissues in which F5H is normally expressed. Finally, we show that repression of both HCE and anthocyanin biosynthesis in fah1 mutants is dependent on the MED5a/5b subunits of the transcriptional coregulatory complex Mediator, which are similarly required for the repression of lignin biosynthesis and the stunted growth of the phenylpropanoid pathway mutant reduced epidermal fluorescence8. Taken together, these observations show that the synthesis of HCEs and anthocyanins is actively repressed in a MEDIATOR-dependent manner in Arabidopsis fah1 mutants and support an emerging model in which MED5a/5b act as central players in the homeostatic repression of phenylpropanoid metabolism.

Research Organization:
Purdue Univ., West Lafayette, IN (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22). Chemical Sciences, Geosciences & Biosciences Division
Grant/Contract Number:
FG02-07ER15905
OSTI ID:
1386267
Alternate ID(s):
OSTI ID: 1597740
Journal Information:
Plant Physiology (Bethesda), Journal Name: Plant Physiology (Bethesda) Vol. 169; ISSN 0032-0889
Publisher:
American Society of Plant BiologistsCopyright Statement
Country of Publication:
United States
Language:
English

References (17)

Redirection of the phenylpropanoid pathway to feruloyl malate in Arabidopsis mutants deficient for cinnamoyl-CoA reductase 1 journal November 2007
Diverse roles of the Mediator complex in plants journal September 2011
Changes in secondary metabolism and deposition of an unusual lignin in the ref8 mutant of Arabidopsis journal April 2002
Visual constraints in foraging bumblebees: Flower size and color affect search time and flight behavior journal March 2001
New routes for lignin biosynthesis defined by biochemical characterization of recombinant ferulate 5-hydroxylase, a multifunctional cytochrome P450-dependent monooxygenase journal August 1999
The Arabidopsis Transcription Factor MYB12 Is a Flavonol-Specific Regulator of Phenylpropanoid Biosynthesis journal May 2005
Sucrose-Specific Induction of the Anthocyanin Biosynthetic Pathway in Arabidopsis journal December 2005
Reprogramming the Phenylpropanoid Metabolism in Seeds of Oilseed Rape by Suppressing the Orthologs of REDUCED EPIDERMAL FLUORESCENCE1 journal February 2013
The Arabidopsis thaliana REDUCED EPIDERMAL FLUORESCENCE1 Gene Encodes an Aldehyde Dehydrogenase Involved in Ferulic Acid and Sinapic Acid Biosynthesis journal January 2004
The sfr6 Mutation in Arabidopsis Suppresses Low-Temperature Induction of Genes Dependent on the CRT/DRE Sequence Motif journal May 1999
The Growth Reduction Associated with Repressed Lignin Biosynthesis in Arabidopsis thaliana Is Independent of Flavonoids journal May 2010
The Arabidopsis Mediator Complex Subunits MED16, MED14, and MED2 Regulate Mediator and RNA Polymerase II Recruitment to CBF-Responsive Cold-Regulated Genes journal January 2014
Activation Tagging Identifies a Conserved MYB Regulator of Phenylpropanoid Biosynthesis journal December 2000
An Arabidopsis mutant defective in the general phenylpropanoid pathway. journal November 1992
Caffeoyl Shikimate Esterase (CSE) Is an Enzyme in the Lignin Biosynthetic Pathway in Arabidopsis journal August 2013
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Cited By (1)

Identification of MOS9 as an interaction partner for chalcone synthase in the nucleus journal January 2018

Figures / Tables (10)

Figure 1(p. 2)figure Figure 1
Figure 2(p. 2)figure Figure 2
Table I(p. 3)table Table I
Figure 3(p. 4)figure Figure 3
Figure 4(p. 4)figure Figure 4
Figure 5(p. 5)figure Figure 5
Figure 6(p. 6)figure Figure 6
Figure 7(p. 7)figure Figure 7
Figure 8(p. 8)figure Figure 8
Figure 9(p. 8)figure Figure 9

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Related Subjects

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