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Title: Loss of ferulate 5-hydroxylase leads to Mediator-dependent inhibition of soluble phenylpropanoid biosynthesis in Arabidopsis

Abstract

Phenylpropanoids are phenylalanine-derived specialized metabolites and include important structural components of plant cell walls, such as lignin and hydroxycinnamic acids, as well as ultraviolet and visible light-absorbing pigments, such as hydroxycinnamate esters (HCEs) and anthocyanins. Previous work has revealed a remarkable degree of plasticity in HCE biosynthesis, such that most Arabidopsis (Arabidopsis thaliana) mutants with blockages in the pathway simply redirect carbon flux to atypical HCEs. In contrast, the ferulic acid hydroxylase1 (fah1) mutant accumulates greatly reduced levels of HCEs, suggesting that phenylpropanoid biosynthesis may be repressed in response to the loss of FERULATE 5-HYDROXYLASE (F5H) activity. Here, we show that in fah1 mutant plants, the activity of HCE biosynthetic enzymes is not limiting for HCE accumulation, nor is phenylpropanoid flux diverted to the synthesis of cell wall components or flavonol glycosides. We further show that anthocyanin accumulation is also repressed in fah1 mutants and that this repression is specific to tissues in which F5H is normally expressed. Finally, we show that repression of both HCE and anthocyanin biosynthesis in fah1 mutants is dependent on the MED5a/5b subunits of the transcriptional coregulatory complex Mediator, which are similarly required for the repression of lignin biosynthesis and the stunted growth of themore » phenylpropanoid pathway mutant reduced epidermal fluorescence8. Taken together, these observations show that the synthesis of HCEs and anthocyanins is actively repressed in a MEDIATOR-dependent manner in Arabidopsis fah1 mutants and support an emerging model in which MED5a/5b act as central players in the homeostatic repression of phenylpropanoid metabolism.« less

Authors:
ORCiD logo [1];  [2];  [3]; ORCiD logo [4]
  1. Purdue Univ., West Lafayette, IN (United States); Heartland Plant Innovations, Manhattan, KS (United States)
  2. Purdue Univ., West Lafayette, IN (United States); Dow AgroSciences LLC, Indianapolis, IN (United States)
  3. Purdue Univ., West Lafayette, IN (United States); Univ. of Wisconsin, Madison, WI (United States)
  4. Purdue Univ., West Lafayette, IN (United States)
Publication Date:
Research Org.:
Purdue Univ., West Lafayette, IN (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22). Chemical Sciences, Geosciences & Biosciences Division; USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
OSTI Identifier:
1386267
Alternate Identifier(s):
OSTI ID: 1597740
Grant/Contract Number:  
SC000997; FG02-07ER15905
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Plant Physiology (Bethesda)
Additional Journal Information:
Journal Volume: 169; Related Information: C3Bio partners with Purdue University (lead); Argonne National Laboratory; National Renewable Energy Laboratory; Northeastern University; University of Tennessee; Journal ID: ISSN 0032-0889
Publisher:
American Society of Plant Biologists
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; catalysis (homogeneous); catalysis (heterogeneous); biofuels (including algae and biomass); bio-inspired; materials and chemistry by design; synthesis (self-assembly); synthesis (scalable processing); 09 BIOMASS FUELS

Citation Formats

Anderson, Nickolas, Bonawitz, Nicholas D., Nyffeler, Kayleigh E., and Chapple, Clint. Loss of ferulate 5-hydroxylase leads to Mediator-dependent inhibition of soluble phenylpropanoid biosynthesis in Arabidopsis. United States: N. p., 2015. Web. doi:10.1104/pp.15.00294.
Anderson, Nickolas, Bonawitz, Nicholas D., Nyffeler, Kayleigh E., & Chapple, Clint. Loss of ferulate 5-hydroxylase leads to Mediator-dependent inhibition of soluble phenylpropanoid biosynthesis in Arabidopsis. United States. doi:10.1104/pp.15.00294.
Anderson, Nickolas, Bonawitz, Nicholas D., Nyffeler, Kayleigh E., and Chapple, Clint. Tue . "Loss of ferulate 5-hydroxylase leads to Mediator-dependent inhibition of soluble phenylpropanoid biosynthesis in Arabidopsis". United States. doi:10.1104/pp.15.00294. https://www.osti.gov/servlets/purl/1386267.
@article{osti_1386267,
title = {Loss of ferulate 5-hydroxylase leads to Mediator-dependent inhibition of soluble phenylpropanoid biosynthesis in Arabidopsis},
author = {Anderson, Nickolas and Bonawitz, Nicholas D. and Nyffeler, Kayleigh E. and Chapple, Clint},
abstractNote = {Phenylpropanoids are phenylalanine-derived specialized metabolites and include important structural components of plant cell walls, such as lignin and hydroxycinnamic acids, as well as ultraviolet and visible light-absorbing pigments, such as hydroxycinnamate esters (HCEs) and anthocyanins. Previous work has revealed a remarkable degree of plasticity in HCE biosynthesis, such that most Arabidopsis (Arabidopsis thaliana) mutants with blockages in the pathway simply redirect carbon flux to atypical HCEs. In contrast, the ferulic acid hydroxylase1 (fah1) mutant accumulates greatly reduced levels of HCEs, suggesting that phenylpropanoid biosynthesis may be repressed in response to the loss of FERULATE 5-HYDROXYLASE (F5H) activity. Here, we show that in fah1 mutant plants, the activity of HCE biosynthetic enzymes is not limiting for HCE accumulation, nor is phenylpropanoid flux diverted to the synthesis of cell wall components or flavonol glycosides. We further show that anthocyanin accumulation is also repressed in fah1 mutants and that this repression is specific to tissues in which F5H is normally expressed. Finally, we show that repression of both HCE and anthocyanin biosynthesis in fah1 mutants is dependent on the MED5a/5b subunits of the transcriptional coregulatory complex Mediator, which are similarly required for the repression of lignin biosynthesis and the stunted growth of the phenylpropanoid pathway mutant reduced epidermal fluorescence8. Taken together, these observations show that the synthesis of HCEs and anthocyanins is actively repressed in a MEDIATOR-dependent manner in Arabidopsis fah1 mutants and support an emerging model in which MED5a/5b act as central players in the homeostatic repression of phenylpropanoid metabolism.},
doi = {10.1104/pp.15.00294},
journal = {Plant Physiology (Bethesda)},
issn = {0032-0889},
number = ,
volume = 169,
place = {United States},
year = {2015},
month = {11}
}

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Cited by: 18 works
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Figures / Tables:

Figure 1 Figure 1: Disruption of phenylpropanoid biosynthetic genes results in the accumulation of normally low-abundance HCEs. Shown are quantifications by HPLC of total HCEs (A) and total flavonol glycosides (B) extracted from 3-week-old whole rosettes of wild-type plants and plants with the indicated mutations. Sinapoylmalate in A is represented in darkmore » gray, and the sum of all other hydroxycinnamoyl esters is represented in light gray. Graphs show means ± SD of three biological replicates.« less

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