Disruption of the endogenous indole glucosinolate pathway impacts the Arabidopsis thaliana root exudation profile and rhizobacterial community

Acuña, Daniel; Bletz, Molly C.; Sasse, Joelle; Micallef, Shirley A.; Kosina, Suzanne; Bowen, Benjamin P.; Northen, Trent R.; Colón-Carmona, Adán

doi:10.1016/j.rhisph.2025.101046

Disruption of the endogenous indole glucosinolate pathway impacts the Arabidopsis thaliana root exudation profile and rhizobacterial community

Journal Article · Tue Feb 11 23:00:00 EST 2025 · Rhizosphere

DOI:https://doi.org/10.1016/j.rhisph.2025.101046· OSTI ID:2557475

Acuña, Daniel ^[1]; Bletz, Molly C. ^[1]; Sasse, Joelle ^[2]; Micallef, Shirley A. ^[3]; Kosina, Suzanne ^[4]; Bowen, Benjamin P. ^[5]; Northen, Trent R. ^[5]; ^[1]

University of Massachusetts, Boston, MA (United States)
USDOE Joint Genome Institute (JGI), Berkeley, CA (United States); University of Zurich (Switzerland)
University of Maryland, College Park, MD (United States)
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States); USDOE Joint Genome Institute (JGI), Berkeley, CA (United States)

Root exudates are composed of primary and secondary metabolites known to modulate the rhizosphere microbiota. Glucosinolates are defense compounds present in the Brassicaceae family capable of deterring pathogens, herbivores and biotic stressors in the phyllosphere. In addition, traces of glucosinolates and their hydrolyzed byproducts have been found in the soil, suggesting that these secondary metabolites could play a role in the modulation and establishment of the rhizosphere microbial community associated with this family. Here, we used Arabidopsis thaliana mutant lines, including the cyp79B2cyp79B3 double mutant line with a disruption in the indole glucosinolate pathway and atr1D, which overexpresses ATR1 and increases glucosinolate production. These lines were analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS) and 16S rRNA amplicon sequencing to evaluate how genetic modifications to the indole glucosinolate pathway affects the root exudate profile of Arabidopsis thaliana, and, in turn, impacts the rhizosphere microbial community. Metabolic analysis of root exudates from the wild-type Columbia (Col-0), along with the mutant lines, confirmed that alterations to the indole glucosinolate biosynthetic pathway result in shifts in the root exudate profile of the plant. We observed changes in the relative abundance of exuded metabolites. Moreover, 16S rRNA amplicon sequencing results provided evidence that the rhizobacterial communities associated with the plant lines used were directly impacted in diversity and community composition. Here, this work provides further information on the involvement of secondary metabolites and their role in modulating the rhizobacterial community. Root metabolites dictate the presence of different bacterial species, including plant growth-promoting rhizobacteria (PGPR). Our results suggest that genetic alterations in the indole glucosinolate pathway cause disruptions beyond the endogenous levels of the plant, significantly changing the abundance and presence of different metabolites in the root exudates of the plants as well as the microbial rhizosphere community.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER). Earth & Environmental Systems Science (EESS); National Science Foundation (NSF)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 2557475

Alternate ID(s):: OSTI ID: 2531011

Journal Information:: Rhizosphere, Journal Name: Rhizosphere Vol. 33; ISSN 2452-2198

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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Disruption of the endogenous indole glucosinolate pathway impacts the Arabidopsis thaliana root exudation profile and rhizobacterial community

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References (75)

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