Sucrose biosensors and methods of using the same

Frommer, Wolf B.; Lager, Ida

Sucrose biosensors and methods of using the same

Patent · Tue May 08 00:00:00 EDT 2012

OSTI ID:1531733

Frommer, Wolf B.; Lager, Ida

Sucrose biosensors are disclosed, which comprise a sucrose binding domain conjugated to donor and fluorescent moieties that permit detection and measurement of Fluorescence Resonance Energy Transfer upon sucrose binding. Such biosensors are useful for real time monitoring of sucrose metabolism in living cells.

View Patent

Research Organization:: Carnegie Institution of Washington, Washington, D.C. (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: FG02-04ER15542

Assignee:: Carnegie Institution of Washington (Washington, DC)

Patent Number(s):: 8,173,863

Application Number:: 12/083,196

OSTI ID:: 1531733

Country of Publication:: United States

Language:: English

References (29)

Glucose Sensor for Low-Cost Lifetime-Based Sensing Using a Genetically Engineered Protein Tolosa, Leah; Gryczynski, Ignacy; Eichhorn, Lisa R. Analytical Biochemistry, Vol. 267, Issue 1 https://doi.org/10.1006/abio.1998.2974	journal	February 1999
Optimized Heterologous Expression of the Polymorphic Human Glutathione Transferase M1-1 Based on Silent Mutations in the Corresponding cDNA Widersten, Mikael; Huang, Mao; Mannervik, Bengt Protein Expression and Purification, Vol. 7, Issue 4 https://doi.org/10.1006/prep.1996.0054	journal	June 1996
Fluorescence resonance energy transfer between blue-emitting and red-shifted excitation derivatives of the green fluorescent protein Mitra, Robi D.; Silva, Christopher M.; Youvan, Douglas C. Gene, Vol. 173, Issue 1 https://doi.org/10.1016/0378-1119(95)00768-7	journal	January 1996
Fusion estrogen receptor proteins: toward the development of receptor-based agonists and antagonists Muyan, Mesut; Yi, Ping; Sathya, Ganesan Molecular and Cellular Endocrinology, Vol. 182, Issue 2 https://doi.org/10.1016/S0303-7207(01)00493-2	journal	September 2001
Enzyme fluorescence as a sensing tool: new perspectives in biotechnology D'Auria, Sabato; Lakowicz, Joseph R. Current Opinion in Biotechnology, Vol. 12, Issue 1 https://doi.org/10.1016/S0958-1669(00)00164-6	journal	February 2001
Building and breeding molecules to spy on cells and tumors Tsien, Roger Y. FEBS Letters, Vol. 579, Issue 4 https://doi.org/10.1016/j.febslet.2004.11.025	journal	November 2004
A novel analytical method for in vivo phosphate tracking Gu, Hong; Lalonde, Sylvie; Okumoto, Sakiko FEBS Letters, Vol. 580, Issue 25 https://doi.org/10.1016/j.febslet.2006.09.048	journal	October 2006
X-ray Structures of the Maltose–Maltodextrin-binding Protein of the Thermoacidophilic Bacterium Alicyclobacillus acidocaldarius Provide Insight into Acid Stability of Proteins Schäfer, Karsten; Magnusson, Ulrika; Scheffel, Frank Journal of Molecular Biology, Vol. 335, Issue 1, p. 261-274 https://doi.org/10.1016/j.jmb.2003.10.042	journal	January 2004
Novel biosensors for the detection of estrogen receptor ligands De, Siddhartha; Macara, Ian G.; Lannigan, Deborah A. The Journal of Steroid Biochemistry and Molecular Biology, Vol. 96, Issue 3-4 https://doi.org/10.1016/j.jsbmb.2005.04.030	journal	August 2005
Periplasmic binding proteins: a versatile superfamily for protein engineering Dwyer, Mary A.; Hellinga, Homme W. Current Opinion in Structural Biology, Vol. 14, Issue 4 https://doi.org/10.1016/j.sbi.2004.07.004	journal	August 2004
Phosphate binding protein as the biorecognition element in a biosensor for phosphate Salins, Lyndon L. E.; Deo, Sapna K.; Daunert, Sylvia Sensors and Actuators B: Chemical, Vol. 97, Issue 1 https://doi.org/10.1016/j.snb.2003.07.019	journal	January 2004
Fluorescence Quenching Studies of Trp Repressor–Operator Interaction Blicharska, Zofia; Wasylewski, Zygmunt Journal of Protein Chemistry, Vol. 18, Issue 8, p. 823-830 https://doi.org/10.1023/A:1020670927293	journal	November 1999
Fluorescent indicators for Ca2+based on green fluorescent proteins and calmodulin Miyawaki, Atsushi; Llopis, Juan; Heim, Roger Nature, Vol. 388, Issue 6645 https://doi.org/10.1038/42264	journal	August 1997
The renaissance of fluorescence resonance energy transfer Selvin, Paul R. Nature Structural Biology, Vol. 7, Issue 9, p. 730-734 https://doi.org/10.1038/78948	journal	September 2000
A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications Nagai, Takeharu; Ibata, Keiji; Park, Eun Sun Nature Biotechnology, Vol. 20, Issue 1 https://doi.org/10.1038/nbt0102-87	journal	January 2002
Detection of glutamate release from neurons by genetically encoded surface-displayed FRET nanosensors Okumoto, S.; Looger, L. L.; Micheva, K. D. Proceedings of the National Academy of Sciences, Vol. 102, Issue 24 https://doi.org/10.1073/pnas.0503274102	journal	June 2005
Visualization of maltose uptake in living yeast cells by fluorescent nanosensors Fehr, M.; Frommer, W. B.; Lalonde, S. Proceedings of the National Academy of Sciences, Vol. 99, Issue 15 https://doi.org/10.1073/pnas.142089199	journal	July 2002
Genetically encoded reporters of protein kinase A activity reveal impact of substrate tethering Zhang, J.; Ma, Y.; Taylor, S. S. Proceedings of the National Academy of Sciences, Vol. 98, Issue 26 https://doi.org/10.1073/pnas.211566798	journal	December 2001
Nucleotide sequence and expression of Escherichia coli trpR, the structural gene for the trp aporepressor. Gunsalus, R. P.; Yanofsky, C. Proceedings of the National Academy of Sciences, Vol. 77, Issue 12 https://doi.org/10.1073/pnas.77.12.7117	journal	December 1980
Kinetic and Thermodynamic Studies of Purine Repressor Binding to Corepressor and Operator DNA Xu, Han; Moraitis, Markos; Reedstrom, Ross J. Journal of Biological Chemistry, Vol. 273, Issue 15 https://doi.org/10.1074/jbc.273.15.8958	journal	April 1998
Fluorescent Biosensor for Quantitative Real-time Measurements of Inositol 1,4,5-Trisphosphate in Single Living Cells Tanimura, Akihiko; Nezu, Akihiro; Morita, Takao Journal of Biological Chemistry, Vol. 279, Issue 37 https://doi.org/10.1074/jbc.C400312200	journal	July 2004
Genetically encoded sensors for ions and metabolites Okumoto, Sakiko; Deuschle, Karen; Fehr, Marcus Soil Science and Plant Nutrition, Vol. 50, Issue 7 https://doi.org/10.1080/00380768.2004.10408560	journal	February 2004
Construction of a fluorescent biosensor family De Lorimier, Robert M.; Smith, J. Jeff; Dwyer, Mary A. Protein Science, Vol. 11, Issue 11 https://doi.org/10.1110/ps.021860	journal	November 2002
Construction and optimization of a family of genetically encoded metabolite sensors by semirational protein engineering Deuschle, Karen; Okumoto, Sakiko; Fehr, Marcus Protein Science, Vol. 14, Issue 9 https://doi.org/10.1110/ps.051508105	journal	September 2005
Protein localization in living cells and tissues using FRET and FLIM Chen, Ye; Mills, James D.; Periasamy, Ammasi Differentiation, Vol. 71, Issue 9-10 https://doi.org/10.1111/j.1432-0436.2003.07109007.x	journal	December 2003
Design of Bioelectronic Interfaces by Exploiting Hinge-Bending Motions in Proteins Benson, D. E. Science, Vol. 293, Issue 5535 https://doi.org/10.1126/science.1062461	journal	August 2001
The Genome of the Natural Genetic Engineer Agrobacterium tumefaciens C58 Wood, D. W. Science, Vol. 294, Issue 5550 https://doi.org/10.1126/science.1066804	journal	December 2001
Shedding Light on Cell Signaling: Interpretation of FRET Biosensors Gaits, F.; Hahn, K. Science Signaling, Vol. 2003, Issue 165 https://doi.org/10.1126/stke.2003.165.pe3	journal	January 2003
Viewpoint: Are Studies in Genetically Altered Mice Out of Control? Sigmund, Curt D. Arteriosclerosis, Thrombosis, and Vascular Biology, Vol. 20, Issue 6 https://doi.org/10.1161/01.ATV.20.6.1425	journal	June 2000

Similar Records

Neurotransmitter sensors and methods of using the same

Patent · Tue Aug 17 00:00:00 EDT 2010 · OSTI ID:1531646

Neurotransmitter sensors and methods of using the same

Patent · Mon Jan 14 23:00:00 EST 2013 · OSTI ID:1531775

Development of sensitive FRET sensors and methods of using the same

Patent · Tue Sep 10 00:00:00 EDT 2013 · OSTI ID:1531872

Sucrose biosensors and methods of using the same

Citation Formats

References (29)

Similar Records

Related Subjects