skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Sucrose biosensors and methods of using the same

Patent ·
OSTI ID:1531733
;

Sucrose biosensors are disclosed, which comprise a sucrose binding domain conjugated to donor and fluorescent moieties that permit detection and measurement of Fluorescence Resonance Energy Transfer upon sucrose binding. Such biosensors are useful for real time monitoring of sucrose metabolism in living cells.

Research Organization:
Carnegie Institution of Washington, Washington, D.C. (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
FG02-04ER15542
Assignee:
Carnegie Institution of Washington (Washington, DC)
Patent Number(s):
8,173,863
Application Number:
12/083,196
OSTI ID:
1531733
Resource Relation:
Patent File Date: 2005-10-14
Country of Publication:
United States
Language:
English

References (42)

Glucose Sensor for Low-Cost Lifetime-Based Sensing Using a Genetically Engineered Protein journal February 1999
Engineered proteins for analyte sensing patent March 2001
Compositions and methods for measuring analyte concentrations patent-application May 2005
Protein localization in living cells and tissues using FRET and FLIM journal December 2003
A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications journal January 2002
Visualization of maltose uptake in living yeast cells by fluorescent nanosensors journal July 2002
Fluorescent indicators for Ca2+based on green fluorescent proteins and calmodulin journal August 1997
Optimized Heterologous Expression of the Polymorphic Human Glutathione Transferase M1-1 Based on Silent Mutations in the Corresponding cDNA journal June 1996
Reductive, energy-transfer fluorogenic probes patent August 1998
Design of Bioelectronic Interfaces by Exploiting Hinge-Bending Motions in Proteins journal August 2001
The Genome of the Natural Genetic Engineer Agrobacterium tumefaciens C58 journal December 2001
Fluorescent protein sensors for detection of analytes patent December 1999
Method and system for rapid biomolecular recognition of amino acids and protein sequencing patent-application May 2002
Enzyme fluorescence as a sensing tool: new perspectives in biotechnology journal February 2001
Sucrose-binding proteins patent-application March 2003
Novel biosensors for the detection of estrogen receptor ligands journal August 2005
X-ray Structures of the Maltose–Maltodextrin-binding Protein of the Thermoacidophilic Bacterium Alicyclobacillus acidocaldarius Provide Insight into Acid Stability of Proteins journal January 2004
Fusion estrogen receptor proteins: toward the development of receptor-based agonists and antagonists journal September 2001
Shedding Light on Cell Signaling: Interpretation of FRET Biosensors journal January 2003
A novel analytical method for in vivo phosphate tracking journal October 2006
Kinetic and Thermodynamic Studies of Purine Repressor Binding to Corepressor and Operator DNA journal April 1998
Phosphate binding protein as the biorecognition element in a biosensor for phosphate journal January 2004
Construction and optimization of a family of genetically encoded metabolite sensors by semirational protein engineering journal September 2005
Fluorescent protein indicators patent October 2002
Fluorescence Quenching Studies of Trp Repressor–Operator Interaction journal November 1999
Tandem fluorescent protein constructs patent November 1999
Construction of a fluorescent biosensor family journal November 2002
Compositions and methods for monitoring the modification of natural binding partners patent October 2002
Monomeric and dimeric fluorescent protein variants and methods for making same patent-application September 2005
Nucleotide sequence and expression of Escherichia coli trpR, the structural gene for the trp aporepressor. journal December 1980
Development Of Sensitive Fret Sensors And Methods Of Using The Same patent-application February 2010
Genetically encoded sensors for ions and metabolites journal February 2004
Detection by fret changes of ligand binding by GFP fusion proteins patent April 2002
Identification of essential genes in microorganisms patent-application February 2004
Building and breeding molecules to spy on cells and tumors journal November 2004
Genetically encoded reporters of protein kinase A activity reveal impact of substrate tethering journal December 2001
Biosensor patent-application June 2004
Detection of glutamate release from neurons by genetically encoded surface-displayed FRET nanosensors journal June 2005
Biosensor patent August 2001
Binding protein as biosensors patent-application July 2003
Periplasmic binding proteins: a versatile superfamily for protein engineering journal August 2004
Fluorescence resonance energy transfer between blue-emitting and red-shifted excitation derivatives of the green fluorescent protein journal January 1996

Similar Records

Related Subjects