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Neurotransmitter sensors and methods of using the same

Patent ·
OSTI ID:1531646
; ;

Neurotransmitter biosensors are disclosed, including YbeJ-based glutamate binding biosensors, comprising a neurotransmitter binding domain conjugated to donor and fluorescent moieties that permit detection and measurement of Fluorescence Resonance Energy Transfer upon binding neurotransmitter. Such biosensors are useful for the detection of neurotransmitter concentrations in vivo and in culture.

Research Organization:
Carnegie Inst. of Washington, Washington, DC (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
FG02-04ER15542
Assignee:
Carnegie Institution of Washington (Washington, DC)
Patent Number(s):
7,777,016
Application Number:
11/665,343
OSTI ID:
1531646
Country of Publication:
United States
Language:
English

References (26)

Detection of glutamate release from neurons by genetically encoded surface-displayed FRET nanosensors journal June 2005
Fluorescent indicators for Ca2+based on green fluorescent proteins and calmodulin journal August 1997
Construction and optimization of a family of genetically encoded metabolite sensors by semirational protein engineering journal September 2005
Building and breeding molecules to spy on cells and tumors journal November 2004
Genetically encoded reporters of protein kinase A activity reveal impact of substrate tethering journal December 2001
Shedding Light on Cell Signaling: Interpretation of FRET Biosensors journal January 2003
Protein localization in living cells and tissues using FRET and FLIM journal December 2003
Glucose Sensor for Low-Cost Lifetime-Based Sensing Using a Genetically Engineered Protein journal February 1999
Design of Bioelectronic Interfaces by Exploiting Hinge-Bending Motions in Proteins journal August 2001
The Genome of the Natural Genetic Engineer Agrobacterium tumefaciens C58 journal December 2001
Domain dislocation: a change of core structure in periplasmic binding proteins in their evolutionary history journal February 1999
Fluorescence Quenching Studies of Trp Repressor–Operator Interaction journal November 1999
Real-Time Characterization of Ribozymes by Fluorecence Resonance Energy Transfer (FRET) journal May 1999
Visualization of maltose uptake in living yeast cells by fluorescent nanosensors journal July 2002
Viewpoint: Are Studies in Genetically Altered Mice Out of Control? journal June 2000
A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications journal January 2002
Nucleotide sequence and expression of Escherichia coli trpR, the structural gene for the trp aporepressor. journal December 1980
X-ray Structures of the Maltose–Maltodextrin-binding Protein of the Thermoacidophilic Bacterium Alicyclobacillus acidocaldarius Provide Insight into Acid Stability of Proteins journal January 2004
Novel biosensors for the detection of estrogen receptor ligands journal August 2005
Fluorescence resonance energy transfer between blue-emitting and red-shifted excitation derivatives of the green fluorescent protein journal January 1996
Fusion estrogen receptor proteins: toward the development of receptor-based agonists and antagonists journal September 2001
Strategies for optimizing heterologous protein expression in Escherichia coli journal February 1998
Construction of a fluorescent biosensor family journal November 2002
Periplasmic binding proteins: a versatile superfamily for protein engineering journal August 2004
Kinetic and Thermodynamic Studies of Purine Repressor Binding to Corepressor and Operator DNA journal April 1998
Optimized Heterologous Expression of the Polymorphic Human Glutathione Transferase M1-1 Based on Silent Mutations in the Corresponding cDNA journal June 1996

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