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Development of sensitive FRET sensors and methods of using the same

Patent ·
OSTI ID:1531872
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Intramolecular biosensors are disclosed, including PBP-based biosensors, comprising a ligand binding domain fused to donor and fluorescent moieties that permit detection and measurement of Fluorescence Resonance Energy Transfer upon binding ligand. At least one of the donor and fluorescent moieties may be internally fused to the biosensor such that both ends of the internally fused fluorophore are fixed. In addition, methods of improving the sensitivity of terminally fused biosensors are provided. The biosensors of the invention are useful for the detection and quantification of ligands in vivo and in culture.

Research Organization:
Carnegie Inst. of Washington, Washington, DC (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
FG02-04ER15542
Assignee:
Carnegie Institution of Washington (Washington, DC)
Patent Number(s):
8,530,633
Application Number:
11/665,339
OSTI ID:
1531872
Country of Publication:
United States
Language:
English

References (31)

Detection of glutamate release from neurons by genetically encoded surface-displayed FRET nanosensors journal June 2005
Fluorescent indicators for Ca2+based on green fluorescent proteins and calmodulin journal August 1997
Construction and optimization of a family of genetically encoded metabolite sensors by semirational protein engineering journal September 2005
Building and breeding molecules to spy on cells and tumors journal November 2004
Enzyme fluorescence as a sensing tool: new perspectives in biotechnology journal February 2001
Genetically encoded reporters of protein kinase A activity reveal impact of substrate tethering journal December 2001
Shedding Light on Cell Signaling: Interpretation of FRET Biosensors journal January 2003
Protein localization in living cells and tissues using FRET and FLIM journal December 2003
Concerted Folding and Binding of a Flexible Colicin Domain to Its Periplasmic Receptor TolA journal April 2003
Glucose Sensor for Low-Cost Lifetime-Based Sensing Using a Genetically Engineered Protein journal February 1999
Design of Bioelectronic Interfaces by Exploiting Hinge-Bending Motions in Proteins journal August 2001
In Vivo Imaging of the Dynamics of Glucose Uptake in the Cytosol of COS-7 Cells by Fluorescent Nanosensors journal March 2003
Fluorescence Quenching Studies of Trp Repressor–Operator Interaction journal November 1999
A novel analytical method for in vivo phosphate tracking journal October 2006
Visualization of maltose uptake in living yeast cells by fluorescent nanosensors journal July 2002
Viewpoint: Are Studies in Genetically Altered Mice Out of Control? journal June 2000
A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications journal January 2002
Genetically encoded sensors for ions and metabolites journal February 2004
Nucleotide sequence and expression of Escherichia coli trpR, the structural gene for the trp aporepressor. journal December 1980
X-ray Structures of the Maltose–Maltodextrin-binding Protein of the Thermoacidophilic Bacterium Alicyclobacillus acidocaldarius Provide Insight into Acid Stability of Proteins journal January 2004
Novel biosensors for the detection of estrogen receptor ligands journal August 2005
Fluorescence resonance energy transfer between blue-emitting and red-shifted excitation derivatives of the green fluorescent protein journal January 1996
Fusion estrogen receptor proteins: toward the development of receptor-based agonists and antagonists journal September 2001
Fluorescence resonance energy transfer in a novel cyclodextrin–Peptide conjugate for detecting steroid molecules journal December 2003
The rational design of allosteric interactions in a monomeric protein and its applications to the construction of biosensors journal April 1997
Periplasmic binding proteins: a versatile superfamily for protein engineering journal August 2004
Kinetic and Thermodynamic Studies of Purine Repressor Binding to Corepressor and Operator DNA journal April 1998
Optical measurement of synaptic glutamate spillover and reuptake by linker optimized glutamate-sensitive fluorescent reporters journal March 2008
Optimized Heterologous Expression of the Polymorphic Human Glutathione Transferase M1-1 Based on Silent Mutations in the Corresponding cDNA journal June 1996
Phosphate binding protein as the biorecognition element in a biosensor for phosphate journal January 2004
Dual-Labeled Glucose Binding Protein for Ratiometric Measurements of Glucose journal January 2004

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