Rapid Ion Mobility Separations of Bile Acid Isomers Using Cyclodextrin Adducts and Structures for Lossless Ion Manipulations

Journal Article · Mon Aug 13 04:00:00 EDT 2018 · Analytical Chemistry

DOI:https://doi.org/10.1021/acs.analchem.8b02990· OSTI ID:1503501

Chouinard, Christopher D. ^[1]; Nagy, Gabe ^[1]; Webb, Ian K. ^[1]; Garimella, Sandilya V. B. ^[1]; Baker, Erin S. ^[1]; Ibrahim, Yehia M. ^[1]; Smith, Richard D. ^[1]

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1. Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington 99352, United States

Bile acids (BAs) constitute an important class of steroid metabolites with changes implicated in disease states and other health conditions. Current analyses for these structurally similar compounds are limited by a lack of sensitivity, and long separation times with often poor isomeric resolution. To overcome these challenges and provide rapid analyses for the BA isomers, we utilized cyclodextrin adducts and ion mobility (IM) separations in structures for lossless ion manipulations (SLIM). Cyclodextrin was found to interact with both the tauro- and glyco-conjugated BA isomers studied, forming rigid noncovalent host-guest inclusion complexes. SLIM capabilities for the accumulation of large ion populations and peak compression, enabled both the sensitive detection and high-resolution separation of all the isomers studied.

• Research Organization: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 1503501
• Report Number(s): PNNL-SA-136059
• Journal Information: Analytical Chemistry, Journal Name: Analytical Chemistry Journal Issue: 18 Vol. 90; ISSN 0003-2700
• Publisher: American Chemical Society (ACS)
• Country of Publication: United States
• Language: English