Distinguishing enantiomeric amino acids with chiral cyclodextrin adducts and structures for lossless ion manipulations

Nagy, Gabe; Chouinard, Christopher D.; Attah, Isaac K.; Webb, Ian K.; Garimella, Sandilya V. B.; Ibrahim, Yehia M.; Baker, Erin S.; Smith, Richard D.

doi:10.1002/elps.201800294

Distinguishing enantiomeric amino acids with chiral cyclodextrin adducts and structures for lossless ion manipulations

Journal Article · Mon Sep 17 00:00:00 EDT 2018 · Electrophoresis

DOI:https://doi.org/10.1002/elps.201800294· OSTI ID:1472151

Nagy, Gabe ^[1]; Chouinard, Christopher D. ^[1]; Attah, Isaac K. ^[1]; Webb, Ian K. ^[1]; Garimella, Sandilya V. B. ^[1]; Ibrahim, Yehia M. ^[1]; Baker, Erin S. ^[1]; ^[1]

Biological Sciences Division Pacific Northwest National Laboratory Richland USA

Abstract

Enantiomeric molecular evaluations remain an enormous challenge for current analytical techniques. To date, derivatization strategies and long separation times are generally required in these studies, and the development and implementation of new approaches are needed to increase speed and distinguish currently unresolvable compounds. Herein, we describe a method using chiral cyclodextrin adducts and structures for lossless ion manipulations (SLIM) and serpentine ultralong path with extended routing (SUPER) ion mobility (IM) to achieve rapid, high resolution separations of d and l enantiomeric amino acids. In the analyses, a chiral cyclodextrin is added to each sample. Two cyclodextrins were found to complex each amino acid molecule (i.e. potentially sandwiching the amino acid in their cavities) and forming host‐guest noncovalent complexes that were distinct for each d and l amino acid pair studied and thus separable with IM in SLIM devices. The SLIM was also used to accumulate much larger ion populations than previously feasible for evaluation and therefore allow enantiomeric measurements of higher sensitivity, with gains in resolution from our ultralong path separation capabilities, than previously reported by any other IM–based approach.

View Accepted Manuscript (Publisher)

Sponsoring Organization:: USDOE

OSTI ID:: 1472151

Alternate ID(s):: OSTI ID: 1496792

Journal Information:: Electrophoresis, Journal Name: Electrophoresis Journal Issue: 24 Vol. 39; ISSN 0173-0835

Publisher:: Wiley Blackwell (John Wiley & Sons)Copyright Statement

Country of Publication:: Germany

Language:: English

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