Effect of in Vivo Deuteration on Structure of Switchgrass Lignin

Meng, Xianzhi; Evans, Barbara R.; Yoo, Chang Geun; Pu, Yunqiao Joseph; Davison, Brian H.; Ragauskas, Arthur J.

doi:10.1021/acssuschemeng.7b01527

Effect of in Vivo Deuteration on Structure of Switchgrass Lignin

Journal Article · Thu Jul 27 00:00:00 EDT 2017 · ACS Sustainable Chemistry & Engineering

DOI:https://doi.org/10.1021/acssuschemeng.7b01527· OSTI ID:1399953

Meng, Xianzhi ^[1]; ^[2]; ^[2]; ^[2]; ^[2]; ^[3]

Univ. of Tennessee, Knoxville, TN (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Univ. of Tennessee, Knoxville, TN (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Biomass deuteration is an effective engineering method that can be used to provide key insights into understanding of biomass recalcitrance and the complex biomass conversion process. In this study, production of deuterated switchgrass was accomplished by growing the plants in 50% D₂O under hydroponic conditions in a perfusion chamber. Cellulolytic enzyme lignin was isolated from deuterated switchgrass, characterized by Fourier transform infrared (FTIR), gel permeation chromatography (GPC), and nuclear magnetic resonance (NMR) and compared with its protiated control sample to determine the effect of in vivo deuteration on the chemical structure of lignin. FTIR results showed that D₂O can be taken up by the roots and transported to the leaves, and deuterium was subsequently incorporated into hydroxyl and alkyl groups in the plant and its lignin through photosynthesis. According to GPC results, deuterated lignin had slightly higher molecular weight, presumably due to isotope effects. ³¹P and heteronuclear single quantum coherence (HSQC) NMR results revealed that lignin in the deuterated biomass preserved its native physicochemical characteristics. Finally, the conserved characteristics of the deuterated lignin show its great potential applications for structural and dynamic studies of lignocellulose by techniques such as neutron scattering.

View Accepted Manuscript (DOE)

Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1399953

Journal Information:: ACS Sustainable Chemistry & Engineering, Journal Name: ACS Sustainable Chemistry & Engineering Journal Issue: 9 Vol. 5; ISSN 2168-0485

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: English

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Ultrastructure and Enzymatic Hydrolysis of Deuterated Switchgrass Bhagia, Samarthya; Meng, Xianzhi; Evans, Barbara R. Scientific Reports, Vol. 8, Issue 1 https://doi.org/10.1038/s41598-018-31269-w	journal	September 2018
Diol pretreatment to fractionate a reactive lignin in lignocellulosic biomass biorefineries Dong, Chengyu; Meng, Xianzhi; Yeung, Chi Shun Green Chemistry, Vol. 21, Issue 10 https://doi.org/10.1039/c9gc00596j	journal	January 2019

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Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
60 APPLIED LIFE SCIENCES
Cellulolytic enzyme lignin
Deuteration
In vivo
Neutron scattering
Switchgrass

Effect of in Vivo Deuteration on Structure of Switchgrass Lignin

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References (34)

Cited By (2)

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