Production of deuterated switchgrass by hydroponic cultivation

Evans, Barbara R.; Bali, Garima; Foston, Marcus B.; Ragauskas, Arthur J.; O'Neill, Hugh Michael; Shah, Riddhi S.; McGaughey, Joseph; Reeves, David T.; Rempe, Caroline S.; Davison, Brian H.

doi:10.1007/s00425-015-2298-0

Production of deuterated switchgrass by hydroponic cultivation

Journal Article · Tue Apr 21 00:00:00 EDT 2015 · Planta

DOI:https://doi.org/10.1007/s00425-015-2298-0· OSTI ID:1185899

Evans, Barbara R. ^[1]; Bali, Garima ^[2]; Foston, Marcus B. ^[3]; Ragauskas, Arthur J. ^[4]; O'Neill, Hugh Michael ^[1]; Shah, Riddhi S. ^[1]; McGaughey, Joseph ^[1]; Reeves, David T. ^[5]; Rempe, Caroline S. ^[6]; Davison, Brian H. ^[1]

Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Georgia Institute of Technology, Atlanta, GA (United States)
Georgia Institute of Technology, Atlanta, GA (United States); Washington Univ., St. Louis, MO (United States)
Georgia Institute of Technology, Atlanta, GA (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Massachusetts, Amherst, MA (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States)

Deuterium enrichment of biological materials can potential enable expanded experimental use of small angle neutron scattering (SANS) to investigate molecular structural transitions of complex systems such as plant cell walls. Two key advances have been made that facilitate cultivation of switchgrass, an important forage and biofuel crop, for controlled isotopic enrichment: (1) perfusion system with individual chambers and (2) hydroponic growth from tiller cuttings. Plants were grown and maintained for several months with periodic harvest. Photosynthetic activity was monitored by measurement of CO₂ in outflow from the growth chambers. Plant morphology and composition appeared normal compared to matched controls grown with H₂O. Using this improved method, gram quantities of switchgrass leaves and stems were produced by continuous hydroponic cultivation using growth medium consisting of basal mineral salts in 50% D₂O. Deuterium incorporation was confirmed by detection of the O-D and C-D stretching peaks with FTIR and quantified by ¹H- and ²H-NMR. Lastly, this capability to produce deuterated lignocellulosic biomass under controlled conditions will enhance investigation of cell wall structure and its deconstruction by neutron scattering and NMR techniques.

View Accepted Manuscript (DOE)

Research Organization:: BioEnergy Science Center (BESC); Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1185899

Journal Information:: Planta, Journal Name: Planta Journal Issue: 1 Vol. 242; ISSN 0032-0935

Publisher:: SpringerCopyright Statement

Country of Publication:: United States

Language:: English

References (22)

Crop Physiology Zegada-Lizarazu, Walter; Wullschleger, Stan D.; Surendran Nair, S. Green Energy and Technology https://doi.org/10.1007/978-1-4471-2903-5_3	book	January 2012
Adaptation and growth response of Arabidopsis thaliana to deuterium Bhatia, C. R.; Smith, H. H. Planta, Vol. 80, Issue 2 https://doi.org/10.1007/BF00385593	journal	January 1968
Changes in lignocellulosic supramolecular and ultrastructure during dilute acid pretreatment of Populus and switchgrass Foston, Marcus; Ragauskas, Art J. Biomass and Bioenergy, Vol. 34, Issue 12 https://doi.org/10.1016/j.biombioe.2010.07.023	journal	December 2010
The effect of deuteration on the structure of bacterial cellulose Bali, Garima; Foston, Marcus B.; O’Neill, Hugh M. Carbohydrate Research, Vol. 374 https://doi.org/10.1016/j.carres.2013.04.009	journal	June 2013
Molecular breeding of switchgrass for use as a biofuel crop Bouton, Joseph H. Current Opinion in Genetics & Development, Vol. 17, Issue 6 https://doi.org/10.1016/j.gde.2007.08.012	journal	December 2007
Breakdown of Cell Wall Nanostructure in Dilute Acid Pretreated Biomass Pingali, Sai Venkatesh; Urban, Volker S.; Heller, William T. Biomacromolecules, Vol. 11, Issue 9 https://doi.org/10.1021/bm100455h	journal	September 2010
Structure Determination of Partially Deuterated Carotenoids from Intrinsically Labeled Vegetables by HPLC-MS and ¹ H NMR Putzbach, Karsten; Krucker, Manfred; Albert, Klaus Journal of Agricultural and Food Chemistry, Vol. 53, Issue 3 https://doi.org/10.1021/jf0487506	journal	February 2005
Effect of D ₂ O on Growth Properties and Chemical Structure of Annual Ryegrass ( Lolium multiflorum ) Evans, Barbara R.; Bali, Garima; Reeves, David T. Journal of Agricultural and Food Chemistry, Vol. 62, Issue 12 https://doi.org/10.1021/jf4055566	journal	March 2014
Germination and Seedling Growth of Winter Rye in Deuterium Oxide Siegel, S. M.; Halpern, L. A.; Giumarro, C. Nature, Vol. 201, Issue 4925 https://doi.org/10.1038/2011244a0	journal	March 1964
Switchgrass as an energy crop for biofuel production: A review of its ligno-cellulosic chemical properties David, Kasi; Ragauskas, Arthur J. Energy & Environmental Science, Vol. 3, Issue 9 https://doi.org/10.1039/b926617h	journal	January 2010
Deuterium incorporation in biomass cell wall components by NMR analysis Foston, Marcus B.; McGaughey, Joseph; O'Neill, Hugh The Analyst, Vol. 137, Issue 5 https://doi.org/10.1039/c2an16025k	journal	January 2012
Estimation of deuteration levels in whole cells and cellular proteins by 1H n.m.r. spectroscopy and neutron scattering Perkins, S. J. Biochemical Journal, Vol. 199, Issue 1 https://doi.org/10.1042/bj1990163	journal	October 1981
Neutron Technologies for Bioenergy Research Langan, Paul; Evans, Barbara R.; Foston, Marcus Industrial Biotechnology, Vol. 8, Issue 4 https://doi.org/10.1089/ind.2012.0012	journal	August 2012
Biomass Characterization: Recent Progress in Understanding Biomass Recalcitrance Foston, Marcus; Ragauskas, Arthur J. Industrial Biotechnology, Vol. 8, Issue 4 https://doi.org/10.1089/ind.2012.0015	journal	August 2012
SANS study of cellulose extracted from switchgrass Pingali, Sai Venkatesh; Urban, Volker S.; Heller, William T. Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 11 https://doi.org/10.1107/S0907444910020408	journal	October 2010
Deuterated Organisms: Cultivation and Uses Katz, J. J.; Crespi, H. L. Science, Vol. 151, Issue 3715 https://doi.org/10.1126/science.151.3715.1187	journal	March 1966
The Biology of Heavy Water Lewis, G. N. Science, Vol. 79, Issue 2042 https://doi.org/10.1126/science.79.2042.151	journal	February 1934
Growth of Roots in Deuterium Oxide Pratt, Robertson; Curry, James American Journal of Botany, Vol. 24, Issue 7 https://doi.org/10.2307/2436423	journal	July 1937
Hydrogen isotope effect on transport of potassium ion in rice seedlings equilibrated with deuterium oxide. Shibabe, Sadami; Yoda, Karin RADIOISOTOPES, Vol. 33, Issue 10 https://doi.org/10.3769/radioisotopes.33.10_675	journal	January 1984
Transport of calcium, germanium and rubidium lons in rice seedlings in deuterium oxide. Shibabe, Sadami; Yoda, Karin RADIOISOTOPES, Vol. 33, Issue 9 https://doi.org/10.3769/radioisotopes.33.9_606	journal	January 1984
Water and potassium ion absorption by deuterium oxide resistant winter rye seedlings. Shibabe, Sadami; Yoda, Karin RADIOISOTOPES, Vol. 34, Issue 5 https://doi.org/10.3769/radioisotopes.34.5_266	journal	January 1985
Golden Rice is an effective source of vitamin A Tang, Guangwen; Qin, Jian; Dolnikowski, Gregory G. The American Journal of Clinical Nutrition, Vol. 89, Issue 6 https://doi.org/10.3945/ajcn.2008.27119	journal	April 2009

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Ultrastructure and Enzymatic Hydrolysis of Deuterated Switchgrass Bhagia, Samarthya; Meng, Xianzhi; Evans, Barbara R. Scientific Reports, Vol. 8, Issue 1 https://doi.org/10.1038/s41598-018-31269-w	journal	September 2018
Additional file 1 of Tracking deuterium uptake in hydroponically grown maize roots using correlative helium ion microscopy and Raman micro-spectroscopy Davoudpour, Yalda; Kümmel, Steffen; Musat, Niculina figshare https://doi.org/10.6084/m9.figshare.23689559	image	January 2023
Additional file 2 of Tracking deuterium uptake in hydroponically grown maize roots using correlative helium ion microscopy and Raman micro-spectroscopy Davoudpour, Yalda; Kümmel, Steffen; Musat, Niculina figshare https://doi.org/10.6084/m9.figshare.23689562.v1	image	January 2023
Additional file 5 of Tracking deuterium uptake in hydroponically grown maize roots using correlative helium ion microscopy and Raman micro-spectroscopy Davoudpour, Yalda; Kümmel, Steffen; Musat, Niculina figshare https://doi.org/10.6084/m9.figshare.23689571.v1	image	January 2023
Production of deuterated biomass by cultivation of Lemna minor (duckweed) in D2O Evans, Barbara R.; Foston, Marcus; O’Neill, Hugh M. Planta, Vol. 249, Issue 5 https://doi.org/10.1007/s00425-019-03097-3	journal	January 2019

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