Hemicellulose characterization of deuterated switchgrass

Bhagia, Samarthya; Pu, Yunqiao; Evans, Barbara R.; Davison, Brian H.; Ragauskas, Arthur J.

doi:10.1016/j.biortech.2018.08.034

Hemicellulose characterization of deuterated switchgrass

Journal Article · Sat Aug 11 00:00:00 EDT 2018 · Bioresource Technology

DOI:https://doi.org/10.1016/j.biortech.2018.08.034· OSTI ID:1468140

Bhagia, Samarthya ^[1]; Pu, Yunqiao ^[2]; Evans, Barbara R. ^[3]; Davison, Brian H. ^[4]; Ragauskas, Arthur J. ^[5]

Univ. of Tennessee, Knoxville, TN (United States). Dept. of Chemical and Biomolecular Engineering
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division. Joint Inst. of Biological Sciences
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Chemical Sciences Division
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division
Univ. of Tennessee, Knoxville, TN (United States). Dept. of Chemical and Biomolecular Engineering. Inst. of Agriculture. Center for Renewable Carbon. Dept. of Forestry, Wildlife, and Fisheries; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division. Joint Inst. of Biological Sciences

This paper describes the structural characterization of hemicellulose isolated from hydroponically grown switchgrass in H₂O medium (protiated) or 50% D₂O medium (deuterated) through compositional analysis, GPC, FTIR, ¹³C and ¹H/¹³C HSQC NMR. 4-O-methyl glucuronoarabinoxylan (GAX), the major hemicellulose in switchgrass isolated from deuterated switchgrass, had structural properties similar to hemicellulose isolated from protiated switchgrass. Both had comparable arabinose to xylose ratio (0.25) and molecular weight (47–50 kDa). Finally, structural similarities show that deuterated switchgrass hemicellulose can be used as a model carbohydrate polymer in neutron scattering, or pharmaceutical studies due to their immunomodulatory activity and gastroprotective effects.

View Accepted Manuscript (DOE)

Research Organization:: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1468140

Journal Information:: Bioresource Technology, Journal Name: Bioresource Technology Vol. 269; ISSN 0960-8524

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
deuterated hemicellulose
deuterated switchgrass
glucuronoarabinoxylan

Hemicellulose characterization of deuterated switchgrass

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