Ultrastructure and Enzymatic Hydrolysis of Deuterated Switchgrass

Bhagia, Samarthya; Meng, Xianzhi; Evans, Barbara R.; Dunlap, John R.; Bali, Garima; Chen, Jihua; Reeves, Kimberly Shawn; Ho, Hoi Chun; Davison, Brian H.; Pu, Yunqiao; Ragauskas, Arthur J.

doi:10.1038/s41598-018-31269-w

Ultrastructure and Enzymatic Hydrolysis of Deuterated Switchgrass

Journal Article · Wed Sep 05 00:00:00 EDT 2018 · Scientific Reports

DOI:https://doi.org/10.1038/s41598-018-31269-w· OSTI ID:1471889

Bhagia, Samarthya ^[1]; Meng, Xianzhi ^[1]; Evans, Barbara R. ^[2]; Dunlap, John R. ^[3]; Bali, Garima ^[4]; Chen, Jihua ^[5]; Reeves, Kimberly Shawn ^[5]; Ho, Hoi Chun ^[6]; Davison, Brian H. ^[7]; Pu, Yunqiao ^[8]; Ragauskas, Arthur J. ^[9]

Univ. of Tennessee, Knoxville, TN (United States). Dept. of Chemical and Biomolecular Engineering
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Chemical Sciences Division
Univ. of Tennessee, Knoxville, TN (United States). Advanced Microscopy and Imaging Center
Georgia Inst. of Technology, Atlanta, GA (United States). Renewable Bioproducts Inst. School of Chemistry and Biochemistry
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Center for Nanophase Materials Sciences
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Carbon and Composite Group. Materials Science and Technology Division; Univ. of Tennessee, Knoxville, TN (United States). The Bredesen Center for Interdisciplinary Research and Graduate Education
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Joint Inst. of Biological Sciences. Biosciences Division
Univ. of Tennessee, Knoxville, TN (United States). Dept. of Chemical and Biomolecular Engineering. Inst. of Agriculture. Center for Renewable Carbon. Dept. of Forestry, Wildlife, and Fisheries; Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Joint Inst. of Biological Sciences. Biosciences Division

Neutron scattering of deuterated plants can provide fundamental insight into the structure of lignocellulosics in plant cell walls and its deconstruction by pretreatment and enzymes. Such plants need to be characterized for any alterations to lignocellulosic structure caused by growth in deuterated media. Here we show that glucose yields from enzymatic hydrolysis at lower enzyme loading were 35% and 30% for untreated deuterated and protiated switchgrass, respectively. Lignin content was 4% higher in deuterated switchgrass but there were no significant lignin structural differences. Transmission electron microscopy showed differences in lignin distribution and packing of fibers in the cell walls that apparently increased surface area of cellulose in deuterated switchgrass, increasing cellulose accessibility and lowering its recalcitrance. These differences in lignification were likely caused by abiotic stress due to growth in deuterated media.

View Accepted Manuscript (DOE)

Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1471889

Journal Information:: Scientific Reports, Journal Name: Scientific Reports Vol. 8; ISSN 2045-2322

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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