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Title: Nanogrid rolling circle DNA sequencing

Abstract

The present invention relates to methods for sequencing a polynucleotide immobilized on an array having a plurality of specific regions each having a defined diameter size, including synthesizing a concatemer of a polynucleotide by rolling circle amplification, wherein the concatemer has a cross-sectional diameter greater than the diameter of a specific region, immobilizing the concatemer to the specific region to make an immobilized concatemer, and sequencing the immobilized concatemer.

Inventors:
; ; ;
Publication Date:
Research Org.:
President and Fellows of Harvard College, Cambridge, MA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1351879
Patent Number(s):
9,624,538
Application Number:
12/120,541
Assignee:
President and Fellows of Harvard College CHO
DOE Contract Number:  
FG02-02ER63445
Resource Type:
Patent
Resource Relation:
Patent File Date: 2008 May 14
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Church, George M., Porreca, Gregory J., Shendure, Jay, and Rosenbaum, Abraham Meir. Nanogrid rolling circle DNA sequencing. United States: N. p., 2017. Web.
Church, George M., Porreca, Gregory J., Shendure, Jay, & Rosenbaum, Abraham Meir. Nanogrid rolling circle DNA sequencing. United States.
Church, George M., Porreca, Gregory J., Shendure, Jay, and Rosenbaum, Abraham Meir. Tue . "Nanogrid rolling circle DNA sequencing". United States. doi:. https://www.osti.gov/servlets/purl/1351879.
@article{osti_1351879,
title = {Nanogrid rolling circle DNA sequencing},
author = {Church, George M. and Porreca, Gregory J. and Shendure, Jay and Rosenbaum, Abraham Meir},
abstractNote = {The present invention relates to methods for sequencing a polynucleotide immobilized on an array having a plurality of specific regions each having a defined diameter size, including synthesizing a concatemer of a polynucleotide by rolling circle amplification, wherein the concatemer has a cross-sectional diameter greater than the diameter of a specific region, immobilizing the concatemer to the specific region to make an immobilized concatemer, and sequencing the immobilized concatemer.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Apr 18 00:00:00 EDT 2017},
month = {Tue Apr 18 00:00:00 EDT 2017}
}

Patent:

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