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Nucleic acid arrays and methods of synthesis

Patent ·
OSTI ID:873967
 [1];  [2];  [3];  [4];  [5]
  1. Allston, MA
  2. Needham, MA
  3. Syracuse, NY
  4. Seattle, WA
  5. Del Mar, CA
+ Show Author Affiliations

The present invention generally relates to high density nucleic acid arrays and methods of synthesizing nucleic acid sequences on a solid surface. Specifically, the present invention contemplates the use of stabilized nucleic acid primer sequences immobilized on solid surfaces, and circular nucleic acid sequence templates combined with the use of isothermal rolling circle amplification to thereby increase nucleic acid sequence concentrations in a sample or on an array of nucleic acid sequences.

Research Organization:
Boston University
DOE Contract Number:
FG02-93ER61609
Assignee:
Trustees of Boston University (Boston, MA)
Patent Number(s):
US 6284497
OSTI ID:
873967
Country of Publication:
United States
Language:
English

References (4)

Specific Enzymatic Amplification of DNA In Vitro: The Polymerase Chain Reaction journal January 1986
Signal amplification of padlock probes by rolling circle replication journal November 1998
Transcription-based amplification system and detection of amplified human immunodeficiency virus type 1 with a bead-based sandwich hybridization format. journal February 1989
Rolling replication of short DNA circles. journal May 1995

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Related Subjects

/435/436/999/
acid
acid arrays
acid sequence
acid sequences
amplification
array
arrays
circle
circular
combined
concentrations
contemplates
density
immobilized
increase
isothermal
methods
nucleic
nucleic acid
primer
relates
rolling
sample
sequence
sequences
solid
solid surface
solid surfaces
specifically
stabilized
surface
surfaces
synthesis
synthesizing
templates