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Title: Nucleic acid arrays and methods of synthesis

Patent ·
OSTI ID:873967
 [1];  [2];  [3];  [4];  [5]
  1. Allston, MA
  2. Needham, MA
  3. Syracuse, NY
  4. Seattle, WA
  5. Del Mar, CA
+ Show Author Affiliations

The present invention generally relates to high density nucleic acid arrays and methods of synthesizing nucleic acid sequences on a solid surface. Specifically, the present invention contemplates the use of stabilized nucleic acid primer sequences immobilized on solid surfaces, and circular nucleic acid sequence templates combined with the use of isothermal rolling circle amplification to thereby increase nucleic acid sequence concentrations in a sample or on an array of nucleic acid sequences.

Research Organization:
Boston University
DOE Contract Number:
FG02-93ER61609
Assignee:
Trustees of Boston University (Boston, MA)
Patent Number(s):
US 6284497
OSTI ID:
873967
Country of Publication:
United States
Language:
English

References (4)

Specific Enzymatic Amplification of DNA In Vitro: The Polymerase Chain Reaction journal January 1986
Signal amplification of padlock probes by rolling circle replication journal November 1998
Rolling replication of short DNA circles. journal May 1995
Transcription-based amplification system and detection of amplified human immunodeficiency virus type 1 with a bead-based sandwich hybridization format. journal February 1989

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Related Subjects

nucleic
acid
arrays
methods
synthesis
relates
density
synthesizing
sequences
solid
surface
specifically
contemplates
stabilized
primer
immobilized
surfaces
circular
sequence
templates
combined
isothermal
rolling
circle
amplification
increase
concentrations
sample
array
solid surface
acid sequence
nucleic acid
acid sequences
solid surfaces
acid arrays
/435/436/999/