Nucleic acid arrays and methods of synthesis

Sabanayagam, Chandran R; Sano, Takeshi; Misasi, John; Hatch, Anson; Cantor, Charles

Title: Nucleic acid arrays and methods of synthesis

Abstract

The present invention generally relates to high density nucleic acid arrays and methods of synthesizing nucleic acid sequences on a solid surface. Specifically, the present invention contemplates the use of stabilized nucleic acid primer sequences immobilized on solid surfaces, and circular nucleic acid sequence templates combined with the use of isothermal rolling circle amplification to thereby increase nucleic acid sequence concentrations in a sample or on an array of nucleic acid sequences.

Inventors:

Sabanayagam, Chandran R ^[1]; Sano, Takeshi ^[2]; Misasi, John ^[3]; Hatch, Anson ^[4]; Cantor, Charles ^[5]

Allston, MA
Needham, MA
Syracuse, NY
Seattle, WA
Del Mar, CA

Publication Date:: Mon Jan 01 00:00:00 EST 2001

Research Org.:: Boston University

OSTI Identifier:: 873967

Patent Number(s):: US 6284497

Assignee:: Trustees of Boston University (Boston, MA)

DOE Contract Number:: FG02-93ER61609

Resource Type:: Patent

Country of Publication:: United States

Language:: English

Subject:: nucleic; acid; arrays; methods; synthesis; relates; density; synthesizing; sequences; solid; surface; specifically; contemplates; stabilized; primer; immobilized; surfaces; circular; sequence; templates; combined; isothermal; rolling; circle; amplification; increase; concentrations; sample; array; solid surface; acid sequence; nucleic acid; acid sequences; solid surfaces; acid arrays; /435/436/999/

Citation Formats


                    Sabanayagam, Chandran R, Sano, Takeshi, Misasi, John, Hatch, Anson, and Cantor, Charles. Nucleic acid arrays and methods of synthesis.  United States: N. p., 2001. 
        Web.

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                    Sabanayagam, Chandran R, Sano, Takeshi, Misasi, John, Hatch, Anson, & Cantor, Charles. Nucleic acid arrays and methods of synthesis.  United States.

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                    Sabanayagam, Chandran R, Sano, Takeshi, Misasi, John, Hatch, Anson, and Cantor, Charles. 2001.  
        "Nucleic acid arrays and methods of synthesis".  United States.  https://www.osti.gov/servlets/purl/873967.

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@article{osti_873967,

  title        = {Nucleic acid arrays and methods of synthesis},

  author       = {Sabanayagam, Chandran R and Sano, Takeshi and Misasi, John and Hatch, Anson and Cantor, Charles},

  abstractNote = {The present invention generally relates to high density nucleic acid arrays and methods of synthesizing nucleic acid sequences on a solid surface. Specifically, the present invention contemplates the use of stabilized nucleic acid primer sequences immobilized on solid surfaces, and circular nucleic acid sequence templates combined with the use of isothermal rolling circle amplification to thereby increase nucleic acid sequence concentrations in a sample or on an array of nucleic acid sequences.},

  doi          = {},

  url          = {https://www.osti.gov/biblio/873967},
  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {Mon Jan 01 00:00:00 EST 2001},

  month        = {Mon Jan 01 00:00:00 EST 2001}

}

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Works referenced in this record:

Specific Enzymatic Amplification of DNA In Vitro: The Polymerase Chain Reaction
journal, January 1986

Mullis, K.; Faloona, F.; Scharf, S.
Cold Spring Harbor Symposia on Quantitative Biology, Vol. 51, Issue 0
https://doi.org/10.1101/SQB.1986.051.01.032

Signal amplification of padlock probes by rolling circle replication
journal, November 1998

Baner, J.; Nilsson, M.; Mendel-Hartvig, M.
Nucleic Acids Research, Vol. 26, Issue 22, p. 5073-5078
https://doi.org/10.1093/nar/26.22.5073

Rolling replication of short DNA circles.
journal, May 1995

Fire, A.; Xu, S. Q.
Proceedings of the National Academy of Sciences, Vol. 92, Issue 10
https://doi.org/10.1073/pnas.92.10.4641

Transcription-based amplification system and detection of amplified human immunodeficiency virus type 1 with a bead-based sandwich hybridization format.
journal, February 1989

Kwoh, D. Y.; Davis, G. R.; Whitfield, K. M.
Proceedings of the National Academy of Sciences, Vol. 86, Issue 4, p. 1173-1177
https://doi.org/10.1073/pnas.86.4.1173

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Similar Records

Title: Nucleic acid arrays and methods of synthesis

Abstract

Citation Formats

Specific Enzymatic Amplification of DNA In Vitro: The Polymerase Chain Reaction journal, January 1986

Signal amplification of padlock probes by rolling circle replication journal, November 1998

Rolling replication of short DNA circles. journal, May 1995

Transcription-based amplification system and detection of amplified human immunodeficiency virus type 1 with a bead-based sandwich hybridization format. journal, February 1989

Specific Enzymatic Amplification of DNA In Vitro: The Polymerase Chain Reaction
journal, January 1986

Signal amplification of padlock probes by rolling circle replication
journal, November 1998

Rolling replication of short DNA circles.
journal, May 1995

Transcription-based amplification system and detection of amplified human immunodeficiency virus type 1 with a bead-based sandwich hybridization format.
journal, February 1989