Expression of eukaryotic polypeptides in chloroplasts
Patent
·
OSTI ID:1176724
The present invention relates to a gene expression system in eukaryotic and prokaryotic cells, preferably plant cells and intact plants. In particular, the invention relates to an expression system having a RB47 binding site upstream of a translation initiation site for regulation of translation mediated by binding of RB47 protein, a member of the poly(A) binding protein family. Regulation is further effected by RB60, a protein disulfide isomerase. The expression system is capable of functioning in the nuclear/cytoplasm of cells and in the chloroplast of plants. Translation regulation of a desired molecule is enhanced approximately 100 fold over that obtained without RB47 binding site activation.
- Research Organization:
- Scripps Research Inst., La Jolla, CA (United States)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- FG03-93ER20116
- Assignee:
- The Scripps Research Institutes (La Jolla, CA)
- Patent Number(s):
- RE44266
- Application Number:
- 11/197,730; 6,156,517
- OSTI ID:
- 1176724
- Resource Relation:
- Patent File Date: 2005 Aug 03
- Country of Publication:
- United States
- Language:
- English
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