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Title: RNA binding protein and binding site useful for expression of recombinant molecules

Abstract

The present invention relates to a gene expression system in eukaryotic and prokaryotic cells, preferably plant cells and intact plants. In particular, the invention relates to an expression system having a RB47 binding site upstream of a translation initiation site for regulation of translation mediated by binding of RB47 protein, a member of the poly(A) binding protein family. Regulation is further effected by RB60, a protein disulfide isomerase. The expression system is capable of functioning in the nuclear/cytoplasm of cells and in the chloroplast of plants. Translation regulation of a desired molecule is enhanced approximately 100 fold over that obtained without RB47 binding site activation.

Inventors:
 [1]
  1. (Cardiff, CA)
Publication Date:
Research Org.:
Scripps Research Institute (La Jolla, CA)
OSTI Identifier:
873426
Patent Number(s):
US 6156517
Assignee:
Scripps Research Institute (La Jolla, CA) OAK
DOE Contract Number:  
FG03-93ER20116
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
rna; binding; protein; site; useful; expression; recombinant; molecules; relates; eukaryotic; prokaryotic; cells; preferably; plant; intact; plants; particular; rb47; upstream; translation; initiation; regulation; mediated; poly; family; effected; rb60; disulfide; isomerase; capable; functioning; nuclear; cytoplasm; chloroplast; desired; molecule; enhanced; approximately; 100; fold; obtained; activation; binding site; binding protein; plant cell; plant cells; recombinant molecule; site useful; /435/999/

Citation Formats

Mayfield, Stephen. RNA binding protein and binding site useful for expression of recombinant molecules. United States: N. p., 2000. Web.
Mayfield, Stephen. RNA binding protein and binding site useful for expression of recombinant molecules. United States.
Mayfield, Stephen. Sat . "RNA binding protein and binding site useful for expression of recombinant molecules". United States. https://www.osti.gov/servlets/purl/873426.
@article{osti_873426,
title = {RNA binding protein and binding site useful for expression of recombinant molecules},
author = {Mayfield, Stephen},
abstractNote = {The present invention relates to a gene expression system in eukaryotic and prokaryotic cells, preferably plant cells and intact plants. In particular, the invention relates to an expression system having a RB47 binding site upstream of a translation initiation site for regulation of translation mediated by binding of RB47 protein, a member of the poly(A) binding protein family. Regulation is further effected by RB60, a protein disulfide isomerase. The expression system is capable of functioning in the nuclear/cytoplasm of cells and in the chloroplast of plants. Translation regulation of a desired molecule is enhanced approximately 100 fold over that obtained without RB47 binding site activation.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Sat Jan 01 00:00:00 EST 2000},
month = {Sat Jan 01 00:00:00 EST 2000}
}

Patent:

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