Expression of eukaryotic polypeptides in chloroplasts

Mayfield, Stephen P

Title: Expression of eukaryotic polypeptides in chloroplasts

Abstract

The present invention relates to a gene expression system in eukaryotic and prokaryotic cells, preferably plant cells and intact plants. In particular, the invention relates to an expression system having a RB47 binding site upstream of a translation initiation site for regulation of translation mediated by binding of RB47 protein, a member of the poly(A) binding protein family. Regulation is further effected by RB60, a protein disulfide isomerase. The expression system is capable of functioning in the nuclear/cytoplasm of cells and in the chloroplast of plants. Translation regulation of a desired molecule is enhanced approximately 100 fold over that obtained without RB47 binding site activation.

Inventors:: Mayfield, Stephen P

Publication Date:: Tue Jun 04 00:00:00 EDT 2013

Research Org.:: The Scripps Research Inst., La Jolla, CA (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1084329

Patent Number(s):: RE44,266

Application Number:: 11/197,730

Assignee:: The Scripps Research Institute (La Jolla, CA)

DOE Contract Number:: FG03-93ER20116

Resource Type:: Patent

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES

Citation Formats


                    Mayfield, Stephen P. Expression of eukaryotic polypeptides in chloroplasts.  United States: N. p., 2013. 
        Web.

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                    Mayfield, Stephen P. Expression of eukaryotic polypeptides in chloroplasts.  United States.

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                    Mayfield, Stephen P. 2013.  
        "Expression of eukaryotic polypeptides in chloroplasts".  United States.  https://www.osti.gov/servlets/purl/1084329.

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@article{osti_1084329,

  title        = {Expression of eukaryotic polypeptides in chloroplasts},

  author       = {Mayfield, Stephen P},

  abstractNote = {The present invention relates to a gene expression system in eukaryotic and prokaryotic cells, preferably plant cells and intact plants. In particular, the invention relates to an expression system having a RB47 binding site upstream of a translation initiation site for regulation of translation mediated by binding of RB47 protein, a member of the poly(A) binding protein family. Regulation is further effected by RB60, a protein disulfide isomerase. The expression system is capable of functioning in the nuclear/cytoplasm of cells and in the chloroplast of plants. Translation regulation of a desired molecule is enhanced approximately 100 fold over that obtained without RB47 binding site activation.},

  doi          = {},

  url          = {https://www.osti.gov/biblio/1084329},
  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {Tue Jun 04 00:00:00 EDT 2013},

  month        = {Tue Jun 04 00:00:00 EDT 2013}

}

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Works referenced in this record:

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Similar Records

Title: Expression of eukaryotic polypeptides in chloroplasts

Abstract

Citation Formats

Construction and characterization of autonomously replicating plasmids in the green unicellular alga Chlamydomonas reinhardii journal, April 1984

Molecular cloning of the beta-subunit of human prolyl 4-hydroxylase. This subunit and protein disulphide isomerase are products of the same gene. journal, March 1987

A poly(A) binding protein functions in the chloroplast as a message-specific translation factor journal, March 1998

Enhancer elements for increased translation in plant plastids patent, August 2001

Studies on Chlamydomonas chloroplast transformation: foreign DNA can be stably maintained in the chromosome. journal, January 1989

Stable chloroplast transformation in potato: use of green fluorescent protein as a plastid marker journal, July 1999

Light regulated translational activators: identification of chloroplast gene specific mRNA binding proteins. journal, December 1991

RNA binding protein and binding site useful for expression of recombinant molecules patent, December 2000

Method to transform algae, materials therefor, and products produced thereby patent, August 1997

ADP-dependent phosphorylation regulates RNA-binding in vitro: implications in light-modulated translation. journal, May 1994

Translational Regulation of Chloroplast Genes journal, January 1996

Transgenic plants expressing a functional single-chain Fv antibody are specifically protected from virus attack journal, December 1993

Molecular cloning of tobacco chromosomal and chloroplast DNA segments capable of replication in yeast journal, October 1983

Protein Disulfide Isomerase as a Regulator of Chloroplast Translational Activation journal, December 1997

Introduction and expression of foreign DNA in isolated spinach chloroplasts by electroporation journal, October 1996

Method for stably transforming plastids of multicellular plants patent, September 1995

Transient foreign gene expression in chloroplasts of cultured tobacco cells after biolistic delivery of chloroplast vectors. journal, January 1990

Genetic engineering of plant chloroplasts patent, December 1997

Translation of the Chloroplast psbA mRNA Requires the Nuclear-encoded Poly(A)-binding Protein, RB47 journal, July 1998

Location and nucleotide sequence of a tobacco chlorophlast DNA segment capable of replication in yeast journal, June 1984

Construction and characterization of autonomously replicating plasmids in the green unicellular alga Chlamydomonas reinhardii
journal, April 1984

Molecular cloning of the beta-subunit of human prolyl 4-hydroxylase. This subunit and protein disulphide isomerase are products of the same gene.
journal, March 1987

A poly(A) binding protein functions in the chloroplast as a message-specific translation factor
journal, March 1998

Enhancer elements for increased translation in plant plastids
patent, August 2001

Studies on Chlamydomonas chloroplast transformation: foreign DNA can be stably maintained in the chromosome.
journal, January 1989

Stable chloroplast transformation in potato: use of green fluorescent protein as a plastid marker
journal, July 1999

Light regulated translational activators: identification of chloroplast gene specific mRNA binding proteins.
journal, December 1991

RNA binding protein and binding site useful for expression of recombinant molecules
patent, December 2000

Method to transform algae, materials therefor, and products produced thereby
patent, August 1997

ADP-dependent phosphorylation regulates RNA-binding in vitro: implications in light-modulated translation.
journal, May 1994

Translational Regulation of Chloroplast Genes
journal, January 1996

Transgenic plants expressing a functional single-chain Fv antibody are specifically protected from virus attack
journal, December 1993

Molecular cloning of tobacco chromosomal and chloroplast DNA segments capable of replication in yeast
journal, October 1983

Protein Disulfide Isomerase as a Regulator of Chloroplast Translational Activation
journal, December 1997

Introduction and expression of foreign DNA in isolated spinach chloroplasts by electroporation
journal, October 1996

Method for stably transforming plastids of multicellular plants
patent, September 1995

Transient foreign gene expression in chloroplasts of cultured tobacco cells after biolistic delivery of chloroplast vectors.
journal, January 1990

Genetic engineering of plant chloroplasts
patent, December 1997

Translation of the Chloroplast psbA mRNA Requires the Nuclear-encoded Poly(A)-binding Protein, RB47
journal, July 1998

Location and nucleotide sequence of a tobacco chlorophlast DNA segment capable of replication in yeast
journal, June 1984