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Title: RNA binding protein and binding site useful for expression of recombinant molecules

Abstract

The present invention relates to a gene expression system in eukaryotic and prokaryotic cells, preferably plant cells and intact plants. In particular, the invention relates to an expression system having a RB47 binding site upstream of a translation initiation site for regulation of translation mediated by binding of RB47 protein, a member of the poly(A) binding protein family. Regulation is further effected by RB60, a protein disulfide isomerase. The expression system is capable of functioning in the nuclear/cytoplasm of cells and in the chloroplast of plants. Translation regulation of a desired molecule is enhanced approximately 100 fold over that obtained without RB47 binding site activation.

Inventors:
Publication Date:
Research Org.:
Scripps Research Institute, San Diego, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1176706
Patent Number(s):
RE39350
Application Number:
10/310,587
Assignee:
Scripps Research Institute
DOE Contract Number:  
FG03-93ER20116
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Mayfield, Stephen P. RNA binding protein and binding site useful for expression of recombinant molecules. United States: N. p., 2006. Web.
Mayfield, Stephen P. RNA binding protein and binding site useful for expression of recombinant molecules. United States.
Mayfield, Stephen P. 2006. "RNA binding protein and binding site useful for expression of recombinant molecules". United States. https://www.osti.gov/servlets/purl/1176706.
@article{osti_1176706,
title = {RNA binding protein and binding site useful for expression of recombinant molecules},
author = {Mayfield, Stephen P.},
abstractNote = {The present invention relates to a gene expression system in eukaryotic and prokaryotic cells, preferably plant cells and intact plants. In particular, the invention relates to an expression system having a RB47 binding site upstream of a translation initiation site for regulation of translation mediated by binding of RB47 protein, a member of the poly(A) binding protein family. Regulation is further effected by RB60, a protein disulfide isomerase. The expression system is capable of functioning in the nuclear/cytoplasm of cells and in the chloroplast of plants. Translation regulation of a desired molecule is enhanced approximately 100 fold over that obtained without RB47 binding site activation.},
doi = {},
url = {https://www.osti.gov/biblio/1176706}, journal = {},
number = ,
volume = ,
place = {United States},
year = {2006},
month = {10}
}

Works referenced in this record: