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Title: Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells

Abstract

This invention provides methods and compositions for incorporation of an unnatural amino acid into a peptide using an orthogonal aminoacyl tRNA synthetase/tRNA pair. In particular, an orthogonal pair is provided to incorporate 5-hydroxy-L-tryptophan in a position encoded by an opal mutation.

Inventors:
; ;
Publication Date:
Research Org.:
The Scripps Research Institute, La Jolla, CA, USA
Sponsoring Org.:
USDOE
OSTI Identifier:
1126420
Patent Number(s):
8,658,395
Application Number:
13/546,582
Assignee:
The Scripps Research Institute (La Jolla, CA) OAK
DOE Contract Number:  
FG03-00ER45812
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES

Citation Formats

Zhang, Zhiwen, Alfonta, Lital, and Schultz, Peter G. Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells. United States: N. p., 2014. Web.
Zhang, Zhiwen, Alfonta, Lital, & Schultz, Peter G. Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells. United States.
Zhang, Zhiwen, Alfonta, Lital, and Schultz, Peter G. Tue . "Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells". United States. https://www.osti.gov/servlets/purl/1126420.
@article{osti_1126420,
title = {Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells},
author = {Zhang, Zhiwen and Alfonta, Lital and Schultz, Peter G},
abstractNote = {This invention provides methods and compositions for incorporation of an unnatural amino acid into a peptide using an orthogonal aminoacyl tRNA synthetase/tRNA pair. In particular, an orthogonal pair is provided to incorporate 5-hydroxy-L-tryptophan in a position encoded by an opal mutation.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2014},
month = {2}
}

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Works referenced in this record:

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