Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells

Zhang, Zhiwen; Alfonta, Lital; Schultz, Peter

Title: Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells

Patent · Tue Jun 22 00:00:00 EDT 2010

OSTI ID:1531637

Zhang, Zhiwen; Alfonta, Lital; Schultz, Peter

This invention provides methods and compositions for incorporation of an unnatural amino acid into a peptide using an orthogonal aminoacyl tRNA synthetase/tRNA pair. In particular, an orthogonal pair is provided to incorporate 5-hydroxy-L-tryptophan in a position encoded by an opal mutation.

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Research Organization:: The Scripps Research Inst., La Jolla, CA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: FG03-00ER45812; NIH GM66494

Assignee:: The Scripps Research Institute (La Jolla, CA)

Patent Number(s):: 7,741,071

Application Number:: 10/580,987

OSTI ID:: 1531637

Resource Relation:: Patent File Date: 2004-12-16

Country of Publication:: United States

Language:: English

References (13)

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In vivo incorporation of unnatural amino acids Schultz, Peter G.; Wang, Lei; Anderson, John Christopher https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7045337 US Patent Document 7,045,337	patent	May 2006
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In vivo incorporation of unnatural amino acids Schultz, Peter; Wang, Lei; Anderson, John Christopher https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20030082575 US Patent Application 10/126927; 20030082575	patent-application	May 2003
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Methods and compositions for the production of orthogonal tRNA-aminoacyltRNA synthetase pairs Schultz, Peter; Wang, Lei; Anderson, John Christopher https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20030108885 US Patent Application 10/126931; 20030108885	patent-application	June 2003
An Expanded Eukaryotic Genetic Code Chin, Jason W.; Cropp, T. Ashton; Anderson, J. Christopher Science, Vol. 301, Issue 5635, p. 964-967 https://doi.org/10.1126/science.1084772	journal	August 2003
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Cited By (3)

Methods and composition for the production of orthogonal tRNA-aminoacyltRNA synthetase pairs Schultz, Peter G.; Wang, Lei; Anderson, John Christopher https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/8183012 US Patent Document 8,183,012	patent	May 2012
Methods and compositions for the production of orthogonal tRNA-aminoacyl tRNA synthetase pairs Schultz, Peter G.; Wang, Lei; Anderson, John Christopher https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/9163271 US Patent Document 9,163,271	patent	October 2015
Expanding the eukaryotic genetic code Chin, Jason W.; Cropp, T. Ashton; Anderson, J. Christopher https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/9580721 US Patent Document 9,580,721	patent	February 2017

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