Genetically programmed expression of proteins containing the unnatural amino acid phenylselenocysteine

Wang, Jiangyun; Schultz, Peter G.

Genetically programmed expression of proteins containing the unnatural amino acid phenylselenocysteine

Patent · Tue Jul 10 00:00:00 EDT 2012

OSTI ID:1176457

Wang, Jiangyun; Schultz, Peter G.

The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate the unnatural amino acid phenylselenocysteine into proteins produced in eubacterial host cells such as E. coli. The invention provides, for example but not limited to, novel orthogonal aminoacyl-tRNA synthetases, polynucleotides encoding the novel synthetase molecules, methods for identifying and making the novel synthetases, methods for producing proteins containing the unnatural amino acid phenylselenocysteine and translation systems. The invention further provides methods for producing modified proteins (e.g., lipidated proteins) through targeted modification of the phenylselenocysteine residue in a protein.

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Research Organization:: The Scripps Research Institute, La Jolla, CA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: FG02-03ER46051; FG03-00ER45812

Assignee:: Scripps Research Institute (La Jolla, CA)

Patent Number(s):: 8,217,145

Application Number:: 12/225,261

OSTI ID:: 1176457

Country of Publication:: United States

Language:: English

References (18)

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