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Title: Genetically programmed expression of proteins containing the unnatural amino acid phenylselenocysteine

Abstract

The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate the unnatural amino acid phenylselenocysteine into proteins produced in eubacterial host cells such as E. coli. The invention provides, for example but not limited to, novel orthogonal aminoacyl-tRNA synthetases, polynucleotides encoding the novel synthetase molecules, methods for identifying and making the novel synthetases, methods for producing proteins containing the unnatural amino acid phenylselenocysteine and translation systems. The invention further provides methods for producing modified proteins (e.g., lipidated proteins) through targeted modification of the phenylselenocysteine residue in a protein.

Inventors:
 [1];  [1]
  1. (La Jolla, CA)
Publication Date:
Research Org.:
The Scripps Research Institute (La Jolla, CA)
Sponsoring Org.:
USDOE
OSTI Identifier:
1014688
Patent Number(s):
7,790,847
Application Number:
US Patent Application 11/724,039
Assignee:
The Scripps Research Institute (La Jolla, CA) OAK
DOE Contract Number:  
FG03-00ER45812
Resource Type:
Patent
Country of Publication:
United States
Language:
English

Citation Formats

Wang, Jiangyun, and Schultz, Peter G. Genetically programmed expression of proteins containing the unnatural amino acid phenylselenocysteine. United States: N. p., 2010. Web.
Wang, Jiangyun, & Schultz, Peter G. Genetically programmed expression of proteins containing the unnatural amino acid phenylselenocysteine. United States.
Wang, Jiangyun, and Schultz, Peter G. Tue . "Genetically programmed expression of proteins containing the unnatural amino acid phenylselenocysteine". United States. https://www.osti.gov/servlets/purl/1014688.
@article{osti_1014688,
title = {Genetically programmed expression of proteins containing the unnatural amino acid phenylselenocysteine},
author = {Wang, Jiangyun and Schultz, Peter G.},
abstractNote = {The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate the unnatural amino acid phenylselenocysteine into proteins produced in eubacterial host cells such as E. coli. The invention provides, for example but not limited to, novel orthogonal aminoacyl-tRNA synthetases, polynucleotides encoding the novel synthetase molecules, methods for identifying and making the novel synthetases, methods for producing proteins containing the unnatural amino acid phenylselenocysteine and translation systems. The invention further provides methods for producing modified proteins (e.g., lipidated proteins) through targeted modification of the phenylselenocysteine residue in a protein.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2010},
month = {9}
}

Patent:

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