Genetically programmed expression of proteins containing the unnatural amino acid phenylselenocysteine

Wang, Jiangyun; Schultz, Peter G

Genetically programmed expression of proteins containing the unnatural amino acid phenylselenocysteine

Patent · Tue Sep 07 00:00:00 EDT 2010

OSTI ID:1014688

Wang, Jiangyun ^[1]; Schultz, Peter G ^[1]

La Jolla, CA

The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate the unnatural amino acid phenylselenocysteine into proteins produced in eubacterial host cells such as E. coli. The invention provides, for example but not limited to, novel orthogonal aminoacyl-tRNA synthetases, polynucleotides encoding the novel synthetase molecules, methods for identifying and making the novel synthetases, methods for producing proteins containing the unnatural amino acid phenylselenocysteine and translation systems. The invention further provides methods for producing modified proteins (e.g., lipidated proteins) through targeted modification of the phenylselenocysteine residue in a protein.

Research Organization:: The Scripps Research Institute (La Jolla, CA)

Sponsoring Organization:: USDOE

DOE Contract Number:: FG03-00ER45812

Assignee:: The Scripps Research Institute (La Jolla, CA)

Patent Number(s):: 7,790,847

Application Number:: US Patent Application 11/724,039

OSTI ID:: 1014688

Country of Publication:: United States

Language:: English

References (13)

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Expanding the Genetic code Wang, Lei; Xie, Jianming; Schultz, Peter G. Annual Review of Biophysics and Biomolecular Structure, Vol. 35, Issue 1 https://doi.org/10.1146/annurev.biophys.35.101105.121507	journal	June 2006
Enzymatic Phosphorylation of Unnatural Nucleosides Wu, Yiqin; Fa, Ming; Tae, Eunju Lee Journal of the American Chemical Society, Vol. 124, Issue 49 https://doi.org/10.1021/ja028050m	journal	December 2002
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Expanding the Genetic Code Wang, Lei; Schultz, Peter G. Angewandte Chemie International Edition, Vol. 44, Issue 1, p. 34-66 https://doi.org/10.1002/anie.200460627	journal	January 2005
A chemical toolkit for proteins — an expanded genetic code Xie, Jianming; Schultz, Peter G. Nature Reviews Molecular Cell Biology, Vol. 7, Issue 10 https://doi.org/10.1038/nrm2005	journal	August 2006
An unnatural base pair for incorporating amino acid analogs into proteins Hirao, Ichiro; Ohtsuki, Takashi; Fujiwara, Tsuyoshi Nature Biotechnology, Vol. 20, Issue 2 https://doi.org/10.1038/nbt0202-177	journal	February 2002
Progress toward the evolution of an organism with an expanded genetic code Liu, David R.; Schultz, Peter G. Proceedings of the National Academy of Sciences, Vol. 96, Issue 9, p. 4780-4785 https://doi.org/10.1073/pnas.96.9.4780	journal	April 1999
Expanding tRNA recognition of a tRNA synthetase by a single amino acid change Feng, L.; Tumbula-Hansen, D.; Toogood, H. Proceedings of the National Academy of Sciences, Vol. 100, Issue 10, p. 5676-5681 https://doi.org/10.1073/pnas.0631525100	journal	May 2003
Programming peptidomimetic syntheses by translating genetic codes designed de novo Forster, A. C.; Tan, Z.; Nalam, M. N. L. Proceedings of the National Academy of Sciences, Vol. 100, Issue 11, p. 6353-6357 https://doi.org/10.1073/pnas.1132122100	journal	May 2003
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Production and fluorescence-activated cell sorting of Escherichia coli expressing a functional antibody fragment on the external surface. Francisco, J. A.; Campbell, R.; Iverson, B. L. Proceedings of the National Academy of Sciences, Vol. 90, Issue 22 https://doi.org/10.1073/pnas.90.22.10444	journal	November 1993

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