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Title: Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells

Patent ·
OSTI ID:1126420
; ;

This invention provides methods and compositions for incorporation of an unnatural amino acid into a peptide using an orthogonal aminoacyl tRNA synthetase/tRNA pair. In particular, an orthogonal pair is provided to incorporate 5-hydroxy-L-tryptophan in a position encoded by an opal mutation.

Research Organization:
The Scripps Research Institute, La Jolla, CA, USA
Sponsoring Organization:
USDOE
DOE Contract Number:
FG03-00ER45812
Assignee:
The Scripps Research Institute (La Jolla, CA)
Patent Number(s):
8,658,395
Application Number:
13/546,582
OSTI ID:
1126420
Country of Publication:
United States
Language:
English

References (21)

In vivo incorporation of unnatural amino acids patent May 2006
In vivo incorporation of unnatural amino acids patent-application May 2003
Methods and compositions for the production of orthogonal tRNA-aminoacyltRNA synthetase pairs patent-application June 2003
Low brain uptake of L-[ 11 C]5-hydroxytryptophan in major depression: a positron emission tomography study on patients and healthy volunteers journal June 1991
Twenty-first aminoacyl-tRNA synthetase-suppressor tRNA pairs for possible use in site-specific incorporation of amino acid analogues into proteins in eukaryotes and in eubacteria journal January 2001
An Expanded Eukaryotic Genetic Code journal August 2003
Cloning and nucleotide sequence of the structural gene coding for Bacillus subtilis tryptophanyl-tRNA synthetase journal December 1988
Aminoacyl-tRNA synthetases journal December 1997
Adding Amino Acids with Novel Reactivity to the Genetic Code of Saccharomyces Cerevisiae
  • Deiters, Alexander; Cropp, T. Ashton; Mukherji, Mridul
  • Journal of the American Chemical Society, Vol. 125, Issue 39, p. 11782-11783 https://doi.org/10.1021/ja0370037
journal October 2003
Protein tolerance to random amino acid change journal June 2004
Characterization of aminoacyl-adenylates in B. tsubtilis tryptophanyl-tRNA synthetase, by the fluorescence of tryptophan analogs 5-hydroxytryptophan and 7-azatryptophan journal December 1993
The renaissance of aminoacyl-tRNA synthesis journal May 2001
A "Silent" Polymorphism in the MDR1 Gene Changes Substrate Specificity journal January 2007
Import of amber and ochre suppressor tRNAs into mammalian cells: A general approach to site-specific insertion of amino acid analogues into proteins journal November 2001
Progress toward the evolution of an organism with an expanded genetic code journal April 1999
Comparative Protein Structure Modeling of Genes and Genomes journal June 2000
A New Orthogonal Suppressor tRNA/Aminoacyl-tRNA Synthetase Pair for Evolving an Organism with an Expanded Genetic Code journal September 2000
Site-specific incorporation of an unnatural amino acid into proteins in mammalian cells journal November 2002
From genes to protein structure and function: novel applications of computational approaches in the genomic era journal January 2000
Identity elements and aminoacylation of plant tRNATrp journal November 1998
A general approach for the generation of orthogonal tRNAs journal September 2001

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