Single nanopore transport of synthetic and biological polyelectrolytes in three-dimensional hybrid microfluidic/nanofluidic devices

King, Travis L.; Gatimu, Enid N.; Bohn, Paul W.

doi:10.1063/1.3059546

Single nanopore transport of synthetic and biological polyelectrolytes in three-dimensional hybrid microfluidic/nanofluidic devices

Journal Article · Thu Jan 01 23:00:00 EST 2009 · Biomicrofluidics

DOI:https://doi.org/10.1063/1.3059546· OSTI ID:1076433

King, Travis L. ^[1]; Gatimu, Enid N. ^[2]; Bohn, Paul W. ^[3]

Univ. of Illinois at Urbana-Champaign, IL (United States). Dept. of Chemistry; Univ. of Notre Dame, South Bend, IN (United States). Dept. of Chemical and Biomolecular Engineering
Univ. of Notre Dame, South Bend, IN (United States). Dept. of Chemical and Biomolecular Engineering
Univ. of Notre Dame, South Bend, IN (United States). Dept. of Chemical and Biomolecular Engineering, Dept. of Chemistry and Biochemistry

This paper presents a study of electrokinetic transport in single nanopores integrated into vertically-stacked three-dimensional hybrid microfluidic/nanofluidic structures. In these devices single nanopores, created by focused ion beam (FIB) milling in thin polymer films, provide fluidic connection between two vertically separated, perpendicular microfluidic channels. Experiments address both systems in which the nanoporous membrane is composed of the same (homojunction) or different (heterojunction) polymer as the microfluidic channels. These devices are then used to study the electrokinetic transport properties of synthetic (i.e., polystyrene sulfonate and polyallylamine) and biological (i.e.,DNA) polyelectrolytes across these nanopores. Single nanopore transport of polyelectrolytes across these nanopores using both electrical current measurements and confocal microscopy. Both optical and electrical measurements indicate that electroosmotic transport is predominant over electrophoresis in single nanopores with d > 180 nm, consistent with results obtained under similar conditions for nanocapillary array membranes.

View Accepted Manuscript (DOE)

Research Organization:: University of Notre Dame, Notre Dame, IN (United States)

Sponsoring Organization:: USDOE SC Office of Biological and Environmental Research (SC-23)

Grant/Contract Number:: FG02-07ER15851

OSTI ID:: 1076433

Journal Information:: Biomicrofluidics, Journal Name: Biomicrofluidics Journal Issue: 1 Vol. 3; ISSN 1932-1058

Publisher:: American Institute of Physics (AIP)Copyright Statement

Country of Publication:: United States

Language:: English

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