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Title: Single nanopore transport of synthetic and biological polyelectrolytes in three-dimensional hybrid microfluidic/nanofluidic devices

Journal Article · · Biomicrofluidics
DOI:https://doi.org/10.1063/1.3059546· OSTI ID:1076433
 [1];  [2];  [3]
  1. Univ. of Illinois at Urbana-Champaign, IL (United States). Dept. of Chemistry; Univ. of Notre Dame, South Bend, IN (United States). Dept. of Chemical and Biomolecular Engineering
  2. Univ. of Notre Dame, South Bend, IN (United States). Dept. of Chemical and Biomolecular Engineering
  3. Univ. of Notre Dame, South Bend, IN (United States). Dept. of Chemical and Biomolecular Engineering, Dept. of Chemistry and Biochemistry
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This paper presents a study of electrokinetic transport in single nanopores integrated into vertically-stacked three-dimensional hybrid microfluidic/nanofluidic structures. In these devices single nanopores, created by focused ion beam (FIB) milling in thin polymer films, provide fluidic connection between two vertically separated, perpendicular microfluidic channels. Experiments address both systems in which the nanoporous membrane is composed of the same (homojunction) or different (heterojunction) polymer as the microfluidic channels. These devices are then used to study the electrokinetic transport properties of synthetic (i.e., polystyrene sulfonate and polyallylamine) and biological (i.e.,DNA) polyelectrolytes across these nanopores. Single nanopore transport of polyelectrolytes across these nanopores using both electrical current measurements and confocal microscopy. Both optical and electrical measurements indicate that electroosmotic transport is predominant over electrophoresis in single nanopores with d > 180 nm, consistent with results obtained under similar conditions for nanocapillary array membranes.

Research Organization:
University of Notre Dame, IN (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
Grant/Contract Number:
FG02-07ER15851
OSTI ID:
1076433
Journal Information:
Biomicrofluidics, Vol. 3, Issue 1; ISSN 1932-1058
Publisher:
American Institute of Physics (AIP)Copyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 15 works
Citation information provided by
Web of Science

References (27)

Design and fabrication of a multilayered polymer microfluidic chip with nanofluidic interconnects via adhesive contact printing journal January 2006
Hybrid three-dimensional nanofluidic/microfluidic devices using molecular gates journal January 2003
Microfluidic Separation and Gateable Fraction Collection for Mass-Limited Samples journal November 2004
Nanocapillary Arrays Effect Mixing and Reaction in Multilayer Fluidic Structures journal March 2004
Nanofluidic Injection and Heterogeneous Kinetics of Organomercaptan Surface Displacement Reactions on Colloidal Gold in a Microfluidic Stream journal April 2006
Integration of nanocapillary arrays into microfluidic devices for use as analyte concentrators journal January 2003
Profiling pH Gradients Across Nanocapillary Array Membranes Connecting Microfluidic Channels journal October 2005
Electrokinetically driven fluidic transport in integrated three-dimensional microfluidic devices incorporating gold-coated nanocapillary array membranes journal January 2008
Direct Immobilization of Fab‘ in Nanocapillaries for Manipulating Mass-Limited Samples journal June 2007
Fabrication of single nanofluidic channels in poly(methylmethacrylate) films via focused-ion beam milling for use as molecular gates journal August 2004
Electrophoresis of a polyelectrolyte through a nanopore journal October 2006
Effect of Salt Concentration on the Electrophoretic Speed of a Polyelectrolyte through a Nanopore journal June 2007
Structure of flexible and semiflexible polyelectrolyte chains in confined spaces of slit micro/nanochannels journal April 2007
Kinetic theory of a confined polymer driven by an external force and pressure-driven flow journal November 2007
Packaging of a Flexible Polyelectrolyte Inside a Viral Capsid:  Effect of Salt Concentration and Salt Valence † journal July 2007
Dynamics of Polyelectrolyte Transport through a Protein Channel as a Function of Applied Voltage journal April 2008
Polyelectrolyte Entry and Transport through an Asymmetric α-Hemolysin Channel journal November 2008
Conformational Analysis of Single DNA Molecules Undergoing Entropically Induced Motion in Nanochannels journal June 2006
Nanopore with transverse nanoelectrodes for electrical characterization and sequencing of DNA journal June 2008
Optical tweezers for force measurements on DNA in nanopores journal October 2006
Single beam optical tweezers setup with backscattered light detection for three-dimensional measurements on DNA and nanopores journal June 2008
Ionic Effects on the Equilibrium Dynamics of DNA Confined in Nanoslits journal May 2008
Size-dependent DNA mobility in nanochannels journal July 2007
Conformation, Length, and Speed Measurements of Electrodynamically Stretched DNA in Nanochannels journal July 2008
Single-file electrophoretic transport and counting of individual DNA molecules in surfactant nanotubes journal June 2005
Refractive index mismatch induced intensity and phase variations in fluorescence confocal, multiphoton and 4Pi-microscopy journal August 1998
Induced Electrokinetic Transport in Micro−Nanofluidic Interconnect Devices journal December 2007

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Development of in-flow label-free single molecule sensors using planar solid-state nanopore integrated microfluidic devices journal September 2018

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