Development of novel separation techniques for biological samples in capillary electrophoresis

Thesis/Dissertation · Wed Jul 27 04:00:00 EDT 1994

DOI:https://doi.org/10.2172/10190677· OSTI ID:10190677

Chang, Huan -Tsung ^[1]

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1. Iowa State Univ., Ames, IA (United States)

This dissertation includes three different topics: general introduction of capillary electrophoresis (CE); gradient in CE and CE in biological separations; and capillary gel electrophoresis (CGE) for DNA separation. Factors such as temperature, viscosity, pH, and the surface of capillary walls affecting the separation performance are demonstrated. A pH gradient between 3.0 and 5.2 is useful to improve the resolution among eight different organic acids. A flow gradient due to the change in the concentration of surfactant, which is able to coat to the capillary wall to change the flow rate and its direction, is also shown as a good way to improve the resolution for organic compounds. A temperature gradient caused by joule heat is shown by voltage programming to enhance the resolution and shorten the separation time for several phenolic compounds. The author also shows that self-regulating dynamic control of electroosmotic flow in CE by simply running separation in different concentrations of surfactant has less matrix effect on the separation performance. One of the most important demonstrations in this dissertation is that the author proposes on-column reaction which gives several advantages including the use of a small amount of sample, low risk of contamination, and time saving and kinetic features. The author uses this idea with laser induced fluorescence (LIF) as a detection mode to detect an on-column digestion of sub-ng of protein. This technique also is applied to single cell analysis in the group.

• Research Organization: Iowa State Univ. of Science and Technology, Ames, IA (United States)
• Sponsoring Organization: USDOE
• DOE Contract Number: W-7405-ENG-82
• OSTI ID: 10190677
• Report Number(s): IS-T--1710; ON: DE95001672
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400105
550200
59 BASIC BIOLOGICAL SCIENCES
BIOCHEMISTRY
DNA
ELECTROPHORESIS
LASER RADIATION
OPTIMIZATION
ORGANIC COMPOUNDS
PERFORMANCE
PROTEINS
SEPARATION PROCEDURES
SEPARATION PROCESSES