Construction and evaluation of a capillary electrophoresis DNA sequencer

Thesis/Dissertation · Tue Dec 31 23:00:00 EST 1991

OSTI ID:7235942

Drossman, H

This dissertation describes the construction and evaluation of an automated DNA sequencer using capillary gel electrophoresis (CGE) for separating single-strand DNA fragments and a fluorescence detector for analyzing labeled fragments. Theories governing the electrophoretic separation of DNA, dispersion processes in CGE and high sensitivity fluorescence detection are reviewed. The CGE DNA sequencer is compared with current DNA sequencing instruments and with projections of future DNA sequencing instruments. Parameters affecting the limits of detection, DNA sample loading, sample mobility and resolution are evaluated. Predictions for the future of capillary electrophoresis for large-scale sequencing projects are presented.

• Research Organization: Wisconsin Univ., Madison, WI (United States)
• OSTI ID: 7235942
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400105* -- Separation Procedures
440800 -- Miscellaneous Instrumentation-- (1990-)
47 OTHER INSTRUMENTATION
AUTOMATION
CAPILLARY FLOW
CONSTRUCTION
DNA SEQUENCERS
ELECTROPHORESIS
EQUIPMENT
FLUID FLOW
LABORATORY EQUIPMENT
PARAMETRIC ANALYSIS