Self-regulating dynamic control of electroosmotic flow in capillary electrophoresis

Journal Article · Sun Feb 28 23:00:00 EST 1993 · Analytical Chemistry (Washington); (United States)

DOI:https://doi.org/10.1021/ac00053a028· OSTI ID:6217327

Chang, Huan-Tsung; Yeung, E S ^[1]

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1. Ames Lab., IA (United States)

Capillary electrophoresis (CE), primarily because of the impressive separation power and the compatibility with large biomolecules, has established itself as an important separation tool over the past several years. One of the major barriers limiting the broad, routine application of the technique is the validation of the reliability of migration times and the quantitative accuracy when dealing with real samples. In particular, sample matrices can alter the nature of the surface of the inner wall of the capillary, leading to irreproducible results. Even when the temperature is constant and when adsorption of the analytes onto the capillary wall can be neglected, which is the case of pure zone electrophoresis (CZE), the [zeta] potential of the wall can still be altered by the injected ions. In this report, the authors demonstrate the utility of a concept for maintaining a constant electroosmotic flow rate for sample ions in CZE, independent of the changing sample matrix composition. Such an interactive control of electroosmotic flow has the advantage that the instantaneous flow rate need not be monitored. Once the stationary front is established, no further external intervention is needed. 23 refs., 2 tabs.

• DOE Contract Number: W-7405-ENG-82
• OSTI ID: 6217327
• Journal Information: Analytical Chemistry (Washington); (United States), Journal Name: Analytical Chemistry (Washington); (United States) Vol. 65:5; ISSN 0003-2700; ISSN ANCHAM
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400105* -- Separation Procedures
400400 -- Electrochemistry
AMMONIUM COMPOUNDS
BLOOD VESSELS
BODY
BROMIDES
BROMINE COMPOUNDS
CAPILLARIES
CAPILLARY FLOW
CARDIOVASCULAR SYSTEM
CONTROL
DATA
DIFFUSION
ELECTROPHORESIS
EXPERIMENTAL DATA
FLUID FLOW
HALIDES
HALOGEN COMPOUNDS
INFORMATION
NUMERICAL DATA
ORGANS
OSMOSIS
SURFACTANTS