Universal nucleic acids sample preparation method for cells, spores and their mixture

Bavykin, Sergei

Universal nucleic acids sample preparation method for cells, spores and their mixture

Patent · Tue Jan 18 04:00:00 EST 2011

OSTI ID:1013054

Bavykin, Sergei ^[1]

Darien, IL

The present invention relates to a method for extracting nucleic acids from biological samples. More specifically the invention relates to a universal method for extracting nucleic acids from unidentified biological samples. An advantage of the presently invented method is its ability to effectively and efficiently extract nucleic acids from a variety of different cell types including but not limited to prokaryotic or eukaryotic cells and/or recalcitrant organisms (i.e. spores). Unlike prior art methods which are focused on extracting nucleic acids from vegetative cell or spores, the present invention effectively extracts nucleic acids from spores, multiple cell types or mixtures thereof using a single method. Important that the invented method has demonstrated an ability to extract nucleic acids from spores and vegetative bacterial cells with similar levels effectiveness. The invented method employs a multi-step protocol which erodes the cell structure of the biological sample, isolates, labels, fragments nucleic acids and purifies labeled samples from the excess of dye.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: W-31109-ENG-38

Assignee:: The United States of America as represented by the United States Department of Energy (Washington, DC)

Patent Number(s):: 7,871,764

Application Number:: 12/050,508

OSTI ID:: 1013054

Country of Publication:: United States

Language:: English

References (14)

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