Detection of nucleic acids by multiple sequential invasive cleavages
Patent
·
OSTI ID:20013245
The present invention relates to methods for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations. The present invention further relates to methods and devices for the separation of nucleic acid molecules based on charge. The present invention also provides methods for the detection of non-target cleavage products via the formation of a complete and activated protein binding region. The invention further provides sensitive and specific methods for the detection of human cytomegalovirus nucleic acid in a sample.
- Research Organization:
- US Department of Energy, Washington, DC (US)
- Sponsoring Organization:
- US Department of Energy
- DOE Contract Number:
- FG02-94ER81891
- Assignee:
- PTO; EDB-00:006667
- Patent Number(s):
- NONE
- Application Number:
- Int. Cl. C12Q 1/68; C12P 19/34; US 5,994,069/A/; 8-823,516
- OSTI ID:
- 20013245
- Country of Publication:
- United States
- Language:
- English
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