Detection of nucleic acids by multiple sequential invasive cleavages

Hall, Jeff G; Lyamichev, Victor I; Mast, Andrea L; Brow, Mary Ann D

Detection of nucleic acids by multiple sequential invasive cleavages

Patent · Fri Jan 01 04:00:00 EST 1999

OSTI ID:872708

Hall, Jeff G ^[1]; Lyamichev, Victor I ^[1]; Mast, Andrea L ^[1]; Brow, Mary Ann D ^[1]

Madison, WI

The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. The present invention further relates to methods and devices for the separation of nucleic acid molecules based on charge. The present invention also provides methods for the detection of non-target cleavage products via the formation of a complete and activated protein binding region. The invention further provides sensitive and specific methods for the detection of human cytomegalovirus nucleic acid in a sample.

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Research Organization:: Third Wave Technologies, Inc., Madison, WI (United States)

DOE Contract Number:: FG02-94ER81891

Assignee:: Third Wave Technologies, Inc. (Madison, WI)

Patent Number(s):: US 5994069

OSTI ID:: 872708

Country of Publication:: United States

Language:: English

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Detection of nucleic acids by multiple sequential invasive cleavages

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