Nucleic acid detection kits

Hall, Jeff G.; Lyamichev, Victor I.; Mast, Andrea L.; Brow, Mary Ann; Kwiatkowski, Robert W.; Vavra, Stephanie H.

Nucleic acid detection kits

Patent · Tue Mar 29 04:00:00 EST 2005

OSTI ID:1175294

Hall, Jeff G.; Lyamichev, Victor I.; Mast, Andrea L.; Brow, Mary Ann; Kwiatkowski, Robert W.; Vavra, Stephanie H.

The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. The present invention further relates to methods and devices for the separation of nucleic acid molecules based on charge. The present invention also provides methods for the detection of non-target cleavage products via the formation of a complete and activated protein binding region. The invention further provides sensitive and specific methods for the detection of nucleic acid from various viruses in a sample.

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Research Organization:: Third Wave Technologies, Inc., Madison, WI, (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: FG02-94ER81891

Assignee:: Third Wave Technologies, Inc. (Madison, WI)

Patent Number(s):: 6,872,816

Application Number:: 09/381,212

OSTI ID:: 1175294

Country of Publication:: United States

Language:: English

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